艾比湖湿地盐节木根际土壤氨氧化微生物多样性和丰度及其与环境因子的相关性分析

发布时间：2018-10-10 06:52

【摘要】：氨氧化反应是硝化作用的关键步骤,参与这一反应的微生物是氨氧化细菌(AOB)和氨氧化古菌(AOA).对新疆艾比湖湿地盐节木根际和非根际土壤的氨氧化微生物进行群落结构和丰度分析,并探究其与土壤理化因子的相关性.同时,以氨单加氧酶基因(amo A)为分子标记,构建克隆文库和测序并与q-PCR法结合研究AOA、AOB的群落结构和丰度,利用Pearson相关分析法探究其与环境因子的相关性.结果表明,根际土壤中AOB的多样性高于AOA,amo A基因序列多属于土壤/水体沉积物分支,AOB克隆文库中的所有序列均属于亚硝化单胞菌属(Nitrosomonas).根际土壤中AOA amo A和AOB amo A的数量分别为2.09×104和2.91×105copies·g~(-1),AOB/AOA的比值为13.9;非根际土壤中AOA amo A和AOB amo A的数量分别为3.85×104和4.76×105copies·g~(-1),AOB/AOA的比值为12.36.相关分析显示,氨氧化微生物的群落结构和丰度与电导率(EC)、有机质(OM)、速效氮(AN)、氨氮(NH_4~+-N)和总氮(TN)等环境因子显著相关.这些结果表明,根际土壤中AOB的群落多样性高于AOA,根际和非根际土壤中AOB的丰度均高于AOA,说明在艾比湖湿地AOB是氨氧化微生物的优势种群,且EC、OM、AN、NH_4~+-N和TN可能会影响氨氧化微生物的群落结构和丰度.
[Abstract]:Ammonia oxidation is a key step in nitrification, and the microbes involved in this reaction are ammonia oxidizing bacteria (AOB) and ammonia-oxidizing palaeomycetes (AOA). The community structure and abundance of ammonia-oxidizing microorganisms in rhizosphere and non-rhizosphere soil of Ebinur Lake wetland in Xinjiang were analyzed and the correlation between them and soil physical and chemical factors was explored. At the same time, the (amo A) gene of ammonia monooxygenase was used as molecular marker to construct the clone library and sequence, and combined with q-PCR method to study the community structure and abundance of AOA,AOB. The correlation between AOA,AOB and environmental factors was explored by Pearson correlation analysis. The results showed that the diversity of AOB in rhizosphere soil was higher than that of AOA,amo A gene sequence. Most of the sequences in soil / water sediment branch AOB clone library belonged to (Nitrosomonas). The ratios of AOA amo A and AOB amo A in rhizosphere soil were 2.09 脳 10 ~ 4 and 2.91 脳 105copies g ~ (-1) AOA, respectively, and those of AOA amo A and AOB amo A in non-rhizosphere soil were 3.85 脳 10 ~ 4 and 4.76 脳 105copies g ~ (-1), respectively. Correlation analysis showed that the community structure and abundance of ammonia-oxidizing microorganisms were significantly correlated with environmental factors such as (EC), organic matter (OM), available nitrogen, (AN), ammonia-N (NH_4~ -N) and total nitrogen (TN). The results showed that the diversity of AOB community in rhizosphere soil was higher than that in AOA, rhizosphere and non-rhizosphere soil, and the abundance of AOB was higher than that of AOA,. The results showed that AOB was the dominant species of ammonia-oxidizing microorganisms in Ebinur Lake wetland. EC,OM,AN,NH_4~-N and TN may affect the community structure and abundance of ammonia-oxidizing microorganisms.
【作者单位】：石河子大学生命科学学院;
【基金】：国家自然科学基金(No.31560040,31160026)~~
【分类号】：S154.3

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