长期种植毛竹林土壤丛枝菌根真菌群落演变趋势

发布时间：2018-10-15 19:59

【摘要】：为揭示长期种植毛竹林土壤丛枝菌根(Arbuscular mycorrhizae,AM)真菌群落演变特征,通过磷脂脂肪酸(Phospholipid fatty acid,PLFA)和Illumina Miseq测序平台研究了AM真菌生物量及群落结构的演变趋势。结果表明,长期种植毛竹林土壤养分含量总体呈下降趋势,球囊霉素相关土壤蛋白(Glomalin-related soil protein,GRSP)含量及AM真菌生物量也显著降低(p0.05),其中易提取态球囊霉素相关土壤蛋白(Easily extractable glomalin-related soil protein,EE-GRSP)含量与有机质、碱解氮、速效钾显著正相关(p0.05),而AM真菌菌丝生物量(16:1ω5 PLFA)与碱解氮极显著正相关(p0.01)。长期种植毛竹林显著降低了土壤2~0.25 mm大团聚体比例(p0.05),且与AM真菌菌丝生物量极显著正相关(p0.01)。测序结果表明,毛竹林土壤AM真菌以球囊霉属(Glomus)为优势种群,其次为无梗囊霉属(Acaulospora),长期种植毛竹后土壤球囊霉属相对丰度显著增加而无梗囊霉属显著降低(p0.05)。非度量多维尺度转换排序(Non-metric multidimensional scaling,NMDS)分析显示,对照马尾松林与不同种植年限毛竹林土壤AM真菌群落显著区分(p=0.001),土壤含水量(p=0.005)、碱解氮(p=0.001)、有效磷(p=0.014)对AM真菌群落结构变异具有重要贡献。长期种植毛竹显著降低了AM真菌生物量、球囊霉素相关土壤蛋白含量以及2~0.25 mm大团聚体比例,并改变了AM真菌群落结构,不利于土壤碳固存和维持生态系统稳定。
[Abstract]:In order to reveal the evolution characteristics of soil arbuscular mycorrhizal (Arbuscular mycorrhizae,AM) fungi community in Phyllostachys pubescens plantation for a long time, the evolution trend of AM fungal biomass and community structure was studied by means of phospholipid fatty acid (Phospholipid fatty acid,PLFA) and Illumina Miseq sequencing platform. The results showed that the soil nutrient content of Phyllostachys pubescens forest decreased in general. The content of balloon mycin associated soil protein (Glomalin-related soil protein,GRSP) and the biomass of AM fungi were also significantly decreased (p0. 05). There was a significant positive correlation between available potassium (p0.05) and mycelium biomass (16:1 蠅 5 PLFA) of AM fungi (p0.01). Long-term cultivation of Phyllostachys pubescens decreased significantly the proportion of large aggregates (p0. 05) of 0.25 mm in soil, and was positively correlated with the mycelial biomass of AM fungi (p0. 01). The results of sequencing showed that the dominant species of AM fungi in the soil of Phyllostachys pubescens was (Glomus), followed by (Acaulospora),. The relative abundance of Bamboo was significantly increased after (Acaulospora), was planted for a long period of time, and the relative abundance of Sporocystis was significantly decreased (p0. 05) in the soil of Phyllostachys pubescens. Non-metric multidimensional scale transformation sorting (Non-metric multidimensional scaling,NMDS) analysis shows that, The soil AM community of Pinus massoniana forest was significantly distinguished from that of Phyllostachys pubescens forest with different planting years (p0. 001), soil water content (p0. 005), alkali-hydrolyzed nitrogen (p0. 001) and available phosphorus (p0. 014) contributed significantly to the structural variation of AM fungal community. Long-term cultivation of Phyllostachys pubescens significantly reduced the biomass of AM fungi, the content of soil protein associated with balloon mycin and the proportion of 2o 0.25 mm aggregates, and changed the community structure of AM fungi, which was not conducive to soil carbon sequestration and maintenance of ecosystem stability.
【作者单位】：浙江农林大学环境与资源学院;浙江省森林生态系统碳循环与固碳减排重点实验室;浙江省临安市农业技术推广中心;
【基金】：国家自然科学基金项目(41671252、41271274) 浙江农林大学农林碳汇与生态环境修复研究中心预研基金项目(2013CB03)资助~~
【分类号】：S714.3

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