枸杞枝条发酵木质纤维素降解与微生物群落多样性研究
发布时间：2018-10-18 08:32
【摘要】：为探讨枸杞枝条基质化发酵中木质纤维素降解、微生物群落代谢能力及多样性特征,采用正交试验设计,以枸杞枝条粉和苦豆子茎秆粉质量比为4∶1混合为试材,采用Biolog-ECO鉴定方法,研究不同发酵因子对枸杞枝条基质化发酵中微生物群落结构和多样性的影响。结果表明:发酵结束时,枸杞枝条基质纤维素、半纤维素和木质素降解率分别在15%、19%及10%以上;木质纤维素降解率在温度为60℃、含水率为60%、添加油饼氮源及接种粗纤维素降解菌处理条件下较高,其中纤维素、半纤维素和木质素降解率分别在18.12%~19.22%、23.55%~25.21%和13.87%~14.24%范围内,显著高于其他处理;该处理增加了枸杞枝条基质发酵高温期时微生物的活性和多样性,平均颜色变化率分别为1.019、1.062、0.943和1.117,微生物多样性香浓指数、优势度指数和丰富度指数分别在2.321~2.365、0.930~0.941和18.78~20.33范围内,提高了枸杞枝条粉堆体中微生物对部分碳源的代谢能力,从而可促使有机质降解。
[Abstract]:In order to study the degradation of lignocellulose and the metabolic ability and diversity of microbial community in the substrate fermentation of Lycium barbarum, the orthogonal design was used. The mixture of Lycium barbarum and Kudou stalk powder was used as the test material at 4:1. The effects of different fermentation factors on the microbial community structure and diversity of Lycium barbarum were studied by Biolog-ECO. The results showed that at the end of fermentation, the degradation rates of cellulose, hemicellulose and lignin in Lycium barbarum branch were over 15% and 10%, respectively, and the degradation rate of lignocellulose was 60 鈩，
本文编号：2278573
[Abstract]:In order to study the degradation of lignocellulose and the metabolic ability and diversity of microbial community in the substrate fermentation of Lycium barbarum, the orthogonal design was used. The mixture of Lycium barbarum and Kudou stalk powder was used as the test material at 4:1. The effects of different fermentation factors on the microbial community structure and diversity of Lycium barbarum were studied by Biolog-ECO. The results showed that at the end of fermentation, the degradation rates of cellulose, hemicellulose and lignin in Lycium barbarum branch were over 15% and 10%, respectively, and the degradation rate of lignocellulose was 60 鈩，
本文编号：2278573
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