连作对再植枸杞根际细菌群落多样性和群落结构的影响

发布时间：2018-10-22 11:57

【摘要】：受枸杞道地产区土地资源等因素限制,连作障碍已成为影响枸杞产业发展的重要原因之一,导致严重的经济损失。研究连作条件下枸杞农田土壤生态系统微生物群落的演替规律对枸杞产业的可持续发展具有重要的理论意义。以宁夏银川市南梁农场连作多年的枸杞地为研究对象,利用Illumina MiSeq测序技术分析了连作对再植枸杞根际/非根际细菌群落的影响。结果表明,连作地显著抑制再植枸杞苗地径的增加,且其土壤pH较对照样地显著降低(p0.05)。测序结果证实,与对照样地相比,连作地再植枸杞根际土壤细菌物种数显著降低(p0.05),细菌群落α多样性下降(p0.05)。主坐标分析表明,连作和对照样地间枸杞非根际细菌群落结构无明显差异,但连作显著改变再植枸杞根际细菌的群落结构。对细菌群落丰度的统计分析发现,连作地枸杞根际浮霉菌门、非根际假单胞菌门的相对丰度较对照样地显著降低(p0.05)。此外,冗余分析结果表明:枸杞园土壤pH和有效磷含量是影响枸杞非根际土壤细菌群落结构变化的主要因素,分别解释了41.8%和35.4%的群落结构变化(p0.05),其他土壤因子无统计学意义,但土壤理化因子对再植枸杞根际细菌群落结构变化的影响均未达显著水平。这些结果证实连作能够显著抑制再植枸杞生长、影响再植枸杞根际细菌群落结构和多样性,干扰枸杞与土壤细菌群落间的互作关系。这些研究结果将为解析枸杞连作障碍机制提供理论基础。
[Abstract]:Restricted by such factors as land resources, continuous cropping obstacles have become one of the important reasons that affect the development of Lycium barbarum industry, resulting in serious economic losses. It is of great theoretical significance to study the succession of microbial communities in the soil ecosystem of Lycium barbarum L. under continuous cropping conditions for the sustainable development of Lycium barbarum industry. The effects of continuous cropping on rhizosphere / non-rhizosphere bacterial communities of Lycium barbarum replanted in Nanliang Farm of Yinchuan City were analyzed by Illumina MiSeq sequencing technique. The results showed that continuous cropping significantly inhibited the increase of Miao Di diameter in replanted Lycium barbarum, and the soil pH decreased significantly (p0.05). The results of sequencing showed that the number of bacterial species in rhizosphere soil of Lycium barbarum replanted under continuous cropping was significantly decreased (p0.05), and the 伪 -diversity of bacterial community was decreased (p0.05). The results of principal coordinate analysis showed that there was no significant difference in the community structure of Lycium barbarum non-rhizosphere bacteria between continuous cropping and intercropping, but the community structure of rhizosphere bacteria in replanted Lycium barbarum was significantly changed by continuous cropping. Statistical analysis of bacterial community abundance showed that the relative abundance of Lycium barbarum rhizosphere floatula phylum and non-rhizosphere Pseudomonas phylum decreased significantly (p0.05). In addition, the results of redundancy analysis showed that the contents of pH and available phosphorus in the soil of Lycium barbarum orchard were the main factors affecting the change of bacterial community structure in non-rhizosphere soil of Lycium barbarum L., which explained 41.8% and 35.4% of the community structure change respectively (p0.05), but other soil factors had no statistical significance. However, the effects of soil physical and chemical factors on the changes of rhizosphere bacterial community structure of Lycium barbarum were not significant. These results showed that continuous cropping could significantly inhibit the growth of replanted Lycium barbarum, affect the structure and diversity of rhizosphere bacterial community of replanted Lycium barbarum, and interfere with the interaction between Lycium barbarum and soil bacterial community. These results will provide a theoretical basis for the analysis of the mechanism of continuous cropping of Lycium barbarum.
【作者单位】：宁夏大学生命科学学院;宁夏农林科学院枸杞研究所;宁夏大学环境工程研究所;
【基金】：国家自然科学基金项目(31560345,31660188)资助~~
【分类号】：S154.36;S567.19

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