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水肥耦合对紫花苜蓿土壤磷酸酶活性的影响

发布时间:2018-11-10 16:41
【摘要】:磷是植物生长的限制因子之一,土壤中占主导地位的有机磷只有在磷酸酶矿化水解作用后才能被作物吸收利用。因此,研究土壤磷酸酶活性特征可为活化土壤中作物较难利用的磷素提供一些途径或方法。本文以紫花苜蓿和栗钙土为研究对象,在盆栽试验条件下分析了不同梯度磷肥施用(P)和水分(W)下的土壤磷酸酶活性特征。结果表明,土壤碱性磷酸单酯酶活性(99.2~170.0 mg para-nitrophenol·kg~(-1) soil·h~(-1))高于酸性磷酸单酯酶活性(24.7~56.9 mg para-nitrophenol·kg~(-1) soil·h~(-1));水肥交互作用对土壤酸性磷酸单酯酶活性和磷酸二酯酶活性均产生显著影响;其中,在常规水分处理条件下,高施磷肥抑制土壤无机焦磷酸酶活性;重度干旱降低了紫花苜蓿土壤酸性磷酸单酯酶、磷酸二酯酶和无机焦磷酸酶活性,但在干旱条件下施磷肥量的增加可提高土壤酸性、碱性磷酸单酯酶活性。因此,通过控制土壤水分和磷肥施用量可达到抑制或激活土壤磷酸酶活性的目的。
[Abstract]:Phosphorus is one of the limiting factors for plant growth. Organic phosphorus, which dominates in soil, can only be absorbed and utilized by crops after mineralized hydrolysis of phosphatase. Therefore, studying the characteristics of soil phosphatase activity can provide some ways or methods for activating phosphorus which is difficult to be used by crops in soil. In this paper, using alfalfa and chestnut soil as research objects, the characteristics of soil phosphatase activity under different gradient phosphate fertilizer application of (P) and water (W) were analyzed in pot experiment. The results showed that the activity of alkaline phosphatase (99.2n 170.0 mg para-nitrophenol kg~ (-1) soil h ~ (-1) was higher than that of acid phosphatase (24.7C 56.9 mg para-nitrophenol kg~ (-1) soil h ~ (-1);). The interaction of water and fertilizer had a significant effect on soil acid phosphatase activity and phosphodiesterase activity, among which high application of phosphate fertilizer inhibited soil inorganic pyrophosphatase activity under conventional water treatment. The activities of acid phosphatase, phosphodiesterase and inorganic pyrophosphatase in alfalfa soil were decreased by severe drought. Therefore, the activity of soil phosphatase can be inhibited or activated by controlling soil moisture and the amount of phosphate fertilizer.
【作者单位】: 赤峰学院资源与环境科学学院;赤峰学院农业科学研究院;东北林业大学林学院;
【基金】:内蒙古自然科学基金(2014BS0302) 国家自然科学基金(31400459)项目资助
【分类号】:S541.9;S154.2

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