云南省粘虫的遗传多样性

发布时间：2018-11-11 17:12

【摘要】：粘虫(Mythimna separata)是重要的季节性迁飞的农业害虫,为弄清云南省粘虫的遗传多样性,本研究利用ISSR分子标记技术分析了云南省23个地理种群粘虫的遗传多样性和遗传结构特征。结果表明:100条ISSR引物中共筛选出9条多态性引物,这些引物在23个地理种群粘虫中扩增出198个位点,各种群多态性位点百分比(P)变化范围为44.95%~66.16%,其中以西畴县西洒镇(XS)种群的多样性位点最高,弥渡(MD)种群最低;种群遗传分化系数(G_(st))为0.3,表明30%的遗传变异发生在种群间,70%的变异来源于相同种群不同个体之间;基因流(N_m)为1.669、平均遗传相似系数为0.898 2,UPGMA聚类分析结果表明23个地理种群遗传距离与地理距离相关性不显著(r=-0.085 6,P=0.282 0)。综合遗传多样性结果,云南省粘虫的遗传多样性较高,各地理种群间产生了一定程度的遗传分化;各种群遗传结构相似性较高,居群间变异较小,居群内变异较大;不存在由遗传漂变引起的种群间遗传分化,该研究结果可为云南省粘虫防控策略的制定提供新的思路和方法。
[Abstract]:(Mythimna separata) is an important seasonal migratory agricultural pest in Yunnan Province. In order to understand the genetic diversity of Mythimycetes in Yunnan Province, the genetic diversity and genetic structure of 23 populations of Yunnan Province were analyzed by ISSR molecular marker technique. The results showed that 9 polymorphic primers were screened out from 100 ISSR primers. These primers amplified 198 loci in 23 geographic populations. The percentage of polymorphic loci (P) varied from 44.95 to 66.16. The diversity of (XS) population was the highest in Xizao Town, Xichou County, and the lowest in Miudu (MD) population. The genetic differentiation coefficient (G _ (st) of the population was 0.3, indicating that 30% of the genetic variation occurred between populations, and 70% of the variation came from different individuals of the same population. The genetic flow (Nm) was 1.669, and the average genetic similarity coefficient was 0.898 (UPGMA). The results showed that there was no significant correlation between genetic distance and geographic distance in 23 populations (r-0.0856 Pu 0.282 0). According to the results of genetic diversity, the genetic diversity of armyworm in Yunnan Province was higher, the genetic structure of different populations was similar, the variation among populations was small, and the variation within populations was higher. There is no genetic differentiation among populations caused by genetic drift. The results of this study can provide a new way of thinking and method for the formulation of control strategy of Mythimygoides in Yunnan Province.
【作者单位】：云南农业大学植物保护学院农业生物多样性与病虫害控制教育部重点实验室;云南省植保植检站;云南省高原特色农业产业研究院;
【基金】：国家自然科学基金项目(31260450,30860069) 云南省科技人才培养计划项目(2013HB072) 云南省植保植检站项目
【分类号】：S433.4

【相似文献】