微生物肥料在不同节肥水平下对烟草根际微生物碳代谢指纹的影响

发布时间：2018-11-13 07:54

【摘要】：为了探究微生物肥料在节肥条件下对烟草根际微生物功能多样性的影响,应用Biolog-ECO技术,研究了金农肥中肥和德根贝在0%、10%、20%、30%节肥水平下烟草根际微生物碳代谢特征。结果表明,金农肥中肥和德根贝的根际微生物,对4类碳源的利用能力均随节肥量的增加而下降;主成分分析表明,在节肥水平下,金农肥中肥和德根贝的根际微生物对碳源的利用方式不同,金农肥中肥偏好代谢中间产物及次生代谢物,而德根贝偏好糖类、脂肪酸和脂类;金农肥中肥和德根贝的根际微生物群落的丰富度、均匀度、优势度在各节肥水平下均显著高于全量施肥(P0.05);从减少化肥施用量对根际微生物碳源代谢能力、碳源利用多样性的影响来看,金农肥中肥和德根贝的节肥比例均以不高于20%为宜,且德根贝表现更优。本研究结果为烟草生产上微生物肥料和节肥能力的选择提供了理论依据。
[Abstract]:In order to investigate the effect of microbial fertilizer on microbial functional diversity in tobacco rhizosphere under the condition of saving fertilizer, Biolog-ECO technique was used to study the effects of Jinnong Fertilizer and Degenbei fertilizer on the functional diversity of tobacco rhizosphere. Carbon Metabolism characteristics of Tobacco rhizosphere microorganisms at 30% Fertilizer level. The results showed that the utilization capacity of four carbon sources decreased with the increase of the amount of fertilizer in Jinnong Fertilizer and the rhizosphere microorganism of Degenbai. Principal component analysis (PCA) showed that in Jinnong Fertilizer and Degenbei fertilizer, the use of carbon sources by rhizosphere microbes was different. Jinnong Fertilizer preferred metabolites and secondary metabolites, while Degenbei preferred carbohydrates, fatty acids and lipids. The richness, evenness and dominance of rhizosphere microbial communities in Jinnong fertilizer and Degenbei fertilizer were significantly higher than those in total fertilization (P0.05). According to the effect of reducing fertilizer application rate on microbial carbon metabolism ability and carbon source utilization diversity in Jinnong Fertilizer, the ratio of saving fertilizer in Jinnong Fertilizer and Degenbei fertilizer was less than 20%, and Degenbai had better performance. The results provide a theoretical basis for the selection of microbial fertilizer and fertilizer saving ability in tobacco production.
【作者单位】：湖南农业大学农学院;江西省红壤研究所;湖南农业大学教育学院;
【基金】：公益性行业(农业)科研专项(201503123-05)
【分类号】：S144;S572

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