苜蓿根系分泌物对土壤难溶性磷活化作用的研究
发布时间：2018-11-15 17:17
【摘要】：选取6个苜蓿品种:骑士2、阿迪娜、TG3、骑士T、中苜1号和标靶,于人工气候箱中采用营养液培养的方法,系统研究了缺磷和供磷条件下,苜蓿生长至60 d、90 d、120 d的根系分泌物对土壤难溶性磷的活化效果及对磷素有效性的影响。结果如下:和对照相比,缺磷条件下,经6个品种苜蓿根系分泌物处理后,土壤中磷素有效性提高;根系分泌物对磷酸二钙(Ca2-P)的活化作用最显著;对磷酸铝盐(Al-P)、磷酸铁盐(Fe-P)和磷酸八钙(Ca8-P)均具有不同程度的活化作用;对磷酸十钙(Ca10-P)和闭蓄态磷(O-P)无显著活化效果。供磷条件下,具有类似的结果,但效果低于缺磷处理。缺磷及供磷条件下,Olsen-P与Ca2-P具有显著或极显著负相关关系,与Al-P具有显著负相关关系,与O-P和Ca10-P无显著相关关系;缺磷条件下与Ca8-P亦显著负相关,与Fe-P相关关系不显著。由此可知,Ca2-P是苜蓿缺磷胁迫下Olsen-P的主要提供者,Al-P、Ca8-P亦可为植株提供Olsen-P,效果低于Ca2-P;Fe-P效果低于Al-P和Ca8-P。和对照相比,土壤经缺磷培养60 d时的苜蓿根系分泌物处理后,Olsen-P含量及难溶性磷含量均有不同程度的变化。其中,TG3处理对土壤Olsen-P含量的提高幅度(11.98%)最为显著,其次是骑士T的7.36%;对土壤Ca2-P的活化能力,表现突出的品种为TG3和骑士T,分别下降了24.99%和24.90%(P0.01),而阿迪娜、中苜1号和标靶对Ca8-P的活化能力低于骑士2、TG3和骑士T。培养至90 d时,骑士T的Olsen-P含量显著(P0.05)高于除中苜1号和标靶外的其他品种;骑士T、中苜1号和标靶等3个品种处理对土壤Ca2-P的活化效果表现突出,土壤中Ca2-P含量除极显著(P0.01)低于对照处理外,亦显著(P0.05)低于其他品种处理;对Fe-P的活化效果表现较好的品种为TG3、骑士T和中苜1号,但和其他品种处理间差异并不显著(P0.05)。120 d时,骑士T、中苜1号和标靶的Olsen-P含量极显著(P0.01)高于对照及其他品种处理;对Ca2-P的活化效果各品种处理均表现突出,极显著低于对照(P0.01);对土壤Ca8-P的活化能力方面,骑士T表现相对较优,但与其他品种处理差异并不显著(P0.05);标靶对Al-P的活化能力表现相对突出,和对照相比其Al-P含量极显著(P0.01)降低了28.49%。供磷条件下,60 d时,TG3处理土壤Olsen-P含量显著(P0.05)高于骑士2、阿迪娜和骑士T;对Ca2-P的活化效果最优的品种为TG3;90 d时,骑士T和中苜1号Olsen-P含量显著(P0.05)高于骑士2和阿迪娜;对Ca2-P的活化效果以骑士T、中苜1号和标靶最好,但各品种之间差异不显著(P0.05);6个品种处理对Al-P、Fe-P和Ca8-P的活化效果无显著性差异(P0.05)。120 d时,骑士T和中苜1号的Olsen-P含量显著(P0.05)高于除标靶外的其他品种;骑士T的Ca2-P含量极显著(P0.01)低于骑士2、阿迪娜和TG3;标靶处理对Al-P的活化效果显著(P0.05)高于除TG3和中苜1号外的其他3个品种处理。同一苜蓿品种,不同培养时间的根系分泌物对土壤Olsen-P含量的影响亦有所不同,表现为120 d90 d60 d,呈现出随生长时间的延长土壤Olsen-P含量提高的趋势。Ca2-P含量则表现为120 d90 d60 d,与60 d相比,缺磷条件培养至120 d时,各品种处理的Ca2-P含量急剧下降,分别为76.34%、74.95%、74.92%、87.06%、88.67%、86.00%(P0.01)。Al-P、Fe-P和Ca8-P的含量变化趋势为120 d90 d60 d。O-P和Ca10-P含量变化无显著规律性。缺磷培养120 d时,苜蓿根系分泌物对Ca2-P、Al-P、Fe-P、Ca8-P的活化效果优于60 d和90 d。由此说明,随着生长天数的增加,根系分泌物对难溶性磷的活化作用愈加显著。苜蓿在供磷和缺磷条件下的根系分泌物对土壤Ca2-P、Ca8-P、Al-P、Fe-P均具有一定的活化作用,缺磷条件下的活化效果高于供磷处理。6个品种中,骑士T根系分泌物相比于其他5个品种对提高土壤Olsen-P含量以及活化土壤中难溶性磷的效果最显著。根系分泌物对土壤Olsen-P的活化量随着植株生长天数的增加而增加,活化效果为120 d90 d60 d。
[Abstract]:Six different varieties were selected: Knights 2, Adina, TG3, Knights T, Zhongjing No. 1 and the target. In the artificial climate box, the method of nutrient solution culture was used. The system studied the growth to 60d and 90d under the condition of phosphorus deficiency and phosphorus supply. The effect of the root exudates of 120d on the soil-insoluble phosphorus and the effect on the effectiveness of the phosphorus. The results were as follows: compared with the control, the effectiveness of the phosphorus in the soil was increased after the treatment of the root exudates of six varieties under the condition of phosphorus deficiency; the activation of the root exudates on the calcium phosphate (Ca2-P) was the most significant; and the aluminum phosphate (Al-P), The iron phosphate (Fe-P) and the calcium phosphate (Ca8-P) have different degree of activation, and no significant activation effect on the calcium phosphate (Ca10-P) and the closed-state phosphorus (O-P). Under the condition of phosphorus supply, similar results are obtained, but the effect is lower than that of the phosphorus-deficient treatment. Under the condition of phosphorus and phosphorus, Olsen-P had a significant or very significant negative correlation with Ca2-P, and there was a significant negative correlation with Al-P, and there was no significant correlation with O-P and Ca10-P. There was a significant negative correlation with Ca8-P under the condition of phosphorus deficiency, and the relationship with Fe-P was not significant. It can be found that Ca2-P is the main provider of Olsen-P under the condition of phosphorus and phosphorus, and the effect of Al-P and Ca8-P can also provide Olsen-P for the plant, and the effect is lower than that of Ca 2-P, and the Fe-P effect is lower than that of Al-P and Ca8-P. Compared with the control, the content of Olsen-P and the content of insoluble phosphorus varied in different degrees after the soil was treated with phosphorus-deficient culture for 60 days. Among them, the increase of the content of Olsen-P in the soil (11.98%) was the most significant, followed by the 7.36% of the Knight's T. The activity of the Ca 2-P in the soil showed that the variety was TG3 and the Knight T, which decreased by 24. 99% and 24. 90% (P0.01). The activation of Ca8-P was lower than that of Knights 2, TG3 and T. The Olsen-P content of the knight T was significantly higher than that of the other species except the medium (1) and the target (P0.05). The results showed that the Ca 2-P content in the soil was significantly lower than that of the control (P <0.01), and the content of Ca 2-P in the soil was lower than that of the control (P <0.01). The results showed that the content of Olsen-P was significantly higher than that of control and other varieties at the time of 120d. The results showed that the treatment of Ca 2-P was significantly lower than that of control (P0.01). The results showed that the activity of Ca8-P in the soil was relatively good, but the difference of the treatment with other varieties was not significant (P0.05); the activation ability of the target to the Al-P was relatively prominent, and the content of Al-P in the control group was significantly lower than that of the control (P0.01). Under the condition of phosphorus supply, the content of Olsen-P in TG3 was higher than that of Knights (P0.05). The effect of Ca 2-P on the activation of Ca 2-P was the best, but the difference between the varieties was not significant (P0.05). The effect of 6 varieties on the activation of Al-P, Fe-P and Ca8-P was not significant (P0.05). The content of Olsen-P was higher than that of the target (P0.05). The content of Ca 2-P was significantly lower than that of Knights 2, Adiana and TG3. The effect of target treatment on the activation of Al-P was significant (P0.05). The effect of root exudates on the content of Olsen-P in the soil was also different, and it was 120 d90 d60 d, which showed a tendency to increase the Olsen-P content in the soil along with the growth time. The content of Ca ~ (2-P) was 120 d90 d60 d, and the content of Ca 2-P in the various varieties decreased sharply, compared with that of 60d. The content of Ca 2-P in the various varieties was 74.34%, 74.95%, 74.92%, 87.6%, 80.67%, 80.00% (P0.01). Al-P, The change of the content of Fe-P and Ca8-P was 120 d90 d60 d. The content of O-P and Ca10-P had no significant regularity. The effect of root exudates on Ca 2-P, Al-P, Fe-P and Ca8-P was better than that of 60d and 90d. The root exudates under the condition of phosphorus and phosphorus deficiency have a certain activation effect on the soil Ca 2-P, Ca8-P, Al-P and Fe-P, and the activation effect under the condition of phosphorus deficiency is higher than that of the phosphorus supply treatment. Compared with the other five varieties, the root exudates of the Knights had the most significant effect on increasing the Olsen-P content of the soil and the hard-soluble phosphorus in the activated soil. The activation of Olsen-P in soil increased with the increase of the number of plant growth days, and the activation effect was 120 d90 d60 d.
【学位授予单位】：河北农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S153.6;S541
本文编号：2333940
[Abstract]:Six different varieties were selected: Knights 2, Adina, TG3, Knights T, Zhongjing No. 1 and the target. In the artificial climate box, the method of nutrient solution culture was used. The system studied the growth to 60d and 90d under the condition of phosphorus deficiency and phosphorus supply. The effect of the root exudates of 120d on the soil-insoluble phosphorus and the effect on the effectiveness of the phosphorus. The results were as follows: compared with the control, the effectiveness of the phosphorus in the soil was increased after the treatment of the root exudates of six varieties under the condition of phosphorus deficiency; the activation of the root exudates on the calcium phosphate (Ca2-P) was the most significant; and the aluminum phosphate (Al-P), The iron phosphate (Fe-P) and the calcium phosphate (Ca8-P) have different degree of activation, and no significant activation effect on the calcium phosphate (Ca10-P) and the closed-state phosphorus (O-P). Under the condition of phosphorus supply, similar results are obtained, but the effect is lower than that of the phosphorus-deficient treatment. Under the condition of phosphorus and phosphorus, Olsen-P had a significant or very significant negative correlation with Ca2-P, and there was a significant negative correlation with Al-P, and there was no significant correlation with O-P and Ca10-P. There was a significant negative correlation with Ca8-P under the condition of phosphorus deficiency, and the relationship with Fe-P was not significant. It can be found that Ca2-P is the main provider of Olsen-P under the condition of phosphorus and phosphorus, and the effect of Al-P and Ca8-P can also provide Olsen-P for the plant, and the effect is lower than that of Ca 2-P, and the Fe-P effect is lower than that of Al-P and Ca8-P. Compared with the control, the content of Olsen-P and the content of insoluble phosphorus varied in different degrees after the soil was treated with phosphorus-deficient culture for 60 days. Among them, the increase of the content of Olsen-P in the soil (11.98%) was the most significant, followed by the 7.36% of the Knight's T. The activity of the Ca 2-P in the soil showed that the variety was TG3 and the Knight T, which decreased by 24. 99% and 24. 90% (P0.01). The activation of Ca8-P was lower than that of Knights 2, TG3 and T. The Olsen-P content of the knight T was significantly higher than that of the other species except the medium (1) and the target (P0.05). The results showed that the Ca 2-P content in the soil was significantly lower than that of the control (P <0.01), and the content of Ca 2-P in the soil was lower than that of the control (P <0.01). The results showed that the content of Olsen-P was significantly higher than that of control and other varieties at the time of 120d. The results showed that the treatment of Ca 2-P was significantly lower than that of control (P0.01). The results showed that the activity of Ca8-P in the soil was relatively good, but the difference of the treatment with other varieties was not significant (P0.05); the activation ability of the target to the Al-P was relatively prominent, and the content of Al-P in the control group was significantly lower than that of the control (P0.01). Under the condition of phosphorus supply, the content of Olsen-P in TG3 was higher than that of Knights (P0.05). The effect of Ca 2-P on the activation of Ca 2-P was the best, but the difference between the varieties was not significant (P0.05). The effect of 6 varieties on the activation of Al-P, Fe-P and Ca8-P was not significant (P0.05). The content of Olsen-P was higher than that of the target (P0.05). The content of Ca 2-P was significantly lower than that of Knights 2, Adiana and TG3. The effect of target treatment on the activation of Al-P was significant (P0.05). The effect of root exudates on the content of Olsen-P in the soil was also different, and it was 120 d90 d60 d, which showed a tendency to increase the Olsen-P content in the soil along with the growth time. The content of Ca ~ (2-P) was 120 d90 d60 d, and the content of Ca 2-P in the various varieties decreased sharply, compared with that of 60d. The content of Ca 2-P in the various varieties was 74.34%, 74.95%, 74.92%, 87.6%, 80.67%, 80.00% (P0.01). Al-P, The change of the content of Fe-P and Ca8-P was 120 d90 d60 d. The content of O-P and Ca10-P had no significant regularity. The effect of root exudates on Ca 2-P, Al-P, Fe-P and Ca8-P was better than that of 60d and 90d. The root exudates under the condition of phosphorus and phosphorus deficiency have a certain activation effect on the soil Ca 2-P, Ca8-P, Al-P and Fe-P, and the activation effect under the condition of phosphorus deficiency is higher than that of the phosphorus supply treatment. Compared with the other five varieties, the root exudates of the Knights had the most significant effect on increasing the Olsen-P content of the soil and the hard-soluble phosphorus in the activated soil. The activation of Olsen-P in soil increased with the increase of the number of plant growth days, and the activation effect was 120 d90 d60 d.
【学位授予单位】：河北农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S153.6;S541
【参考文献】
相关期刊论文 前2条
1 李勇;黄小芳;丁万隆;;根系分泌物及其对植物根际土壤微生态环境的影响[J];华北农学报;2008年S1期
2 周冀衡,李永平,杨虹琦,王勇,邵岩,郭紫明,朱列书,马文广;不同基因型烟草根系分泌物对难溶性磷钾的活化效应[J];湖南农业大学学报(自然科学版);2005年03期
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