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碳氮比对猪粪和稻秆协同生物降解的影响

发布时间:2018-11-19 08:14
【摘要】:农业废弃物猪粪和稻秆产量巨大,因缺乏有效的处置方式,从而造成了巨大的污染,阻碍了现代农业的可持续发展。本研究以猪粪和稻秆的协同高效降解为研究导向,首次采用纤维素分解复合菌系预处理技术进行协同降解。通过考察碳氮比为10∶1、13∶1、16∶1和19∶1等4个梯度下的猪粪和稻秆混合物协同降解情况,并测定不同碳氮比不同培养时间下降解液的关键参数,探讨了不同碳氮比条件下复合菌系对农业废弃物协同降解效果的影响。结果表明,该菌系在碳氮比为16∶1、固形物含量为6%、接种量为10%的条件下于55℃下培养96 h,对猪粪和稻秆混合物的降解效果最佳。在此条件下其失重率高达26.2%,降解过程中的关键酶——滤纸酶和羧甲基纤维素酶(CMC酶)酶活可分别达到3.2和10.8 IU。该复合菌系在对猪粪和稻秆混合物协同降解过程中除了添加少许氯化钠外,无需再额外添加其他营养物质,展现出了良好的实际应用前景,可进一步为其他种类的农业废弃物的高效生物处置提供一定的理论依据。
[Abstract]:The yield of pig dung and rice straw is huge, which causes huge pollution and hinders the sustainable development of modern agriculture because of the lack of effective disposal methods. In this study, the synergistic degradation of pig manure and rice stalk was studied, and the pretreatment technology of cellulose decomposing complex strain was used for the first time. The synergistic degradation of pig dung and rice straw mixtures with C / N ratio of 10: 110: 1: 16: 1 and 19:1 was investigated, and the key parameters of degradation solution under different C / N ratios and different incubation time were determined. The effects of compound strains on the synergistic degradation of agricultural wastes under different C / N ratios were studied. The results showed that under the conditions of C / N ratio of 16: 1, solid content of 6 and inoculation amount of 10%, the strain was cultured at 55 鈩,

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