广州地区丽赤壳属真菌的种类鉴定
发布时间：2018-11-19 08:23
【摘要】：丽赤壳属(Calonectria)真菌是一类重要的植物病原菌,其分布范围广,可为害林木及农作物等,造成严重的经济损失。广东地处南亚热带季风气候区,气候温暖潮湿,丽赤壳属真菌所致病害严重。为明确丽赤壳属种类,为广州市丽赤壳属真菌病害防控提供理论依据,本论文对广州及周边地区的丽赤壳属真菌进行了调查采样和分离,通过形态学和分子生物学方法对分离获得的菌株进行了种类鉴定,并分析其系统发育关系,主要研究结果如下:从广州地区的植物上共采集分离得到16个丽赤壳属真菌菌株。在观察标准培养条件下形态学特征的基础上,结合肌动蛋白(ACT)、钙调蛋白(CAL)、组蛋白H3(HIS3)、翻译延伸因子(TEF-1α)和β-微管蛋白(TUB2)共5个基因序列的系统发育分析,将16个菌株鉴定为5个复合群中11个种:Calonectria cylindrospora复合群中的天南星生丽赤壳Ca.spathiphyllicola、红千层丽赤壳Ca.callistemonii、天南星丽赤壳Ca.spathiphylli、霍奇斯丽赤壳Ca.hodgesii;Ca.brassicae复合群中的东方丽赤壳Ca.orientalis;Ca.reteaudii复合群中的野牡丹丽赤壳Ca.tibouchinae、五隔大无性孢丽赤壳Ca.pentaseptata、小无性孢丽赤壳Ca.microconidialis;Ca.colhounii复合群中的克儒斯丽赤壳Ca.crousiana;Ca.kyotensis复合群中的香港丽赤壳Ca.hongkongensis、冬青丽赤壳Ca.ilicicola。其中,Ca.spathiphyllicola、Ca.callistemonii、Ca.tibouchinae为新种,Ca.orientalis和Ca.hodgesii为中国新记录种。签名树(Clusia rosea)、野牡丹(Melastoma sp.)为丽赤壳属真菌新寄主,蔷薇(Rosa sp.)为Ca.hodgesii新寄主,红千层(Callistemon rigidus)为Ca.pentaseptata、Ca.crousiana新寄主,千层金(Melaleuca bracteata)为Ca.pentaseptata新寄主,鹤望兰(Strelitzia reginae)为Ca.microconidialis新寄主,番荔枝(Annona squamosa)和凤梨(Ananas sp.)为Ca.ilicicola新寄主。所鉴定菌株均附有菌落特征、显微形态图和形态特征描述。研究发现,16个丽赤壳属菌株形态存在丰富的多样性,仅依靠形态特征难以正确区分其种类。本研究通过形态学结合ACT、HIS3、TEF-1α、CAL和TUB2等5个基因联合序列系统发育树分析,能有效地将16个菌株鉴定到种的水平。5个基因中,ACT基因对丽赤壳属真菌物种的鉴别能力最弱,而TEF-1α基因对复合群中相似种的划分最有效。
[Abstract]:(Calonectria) fungi are a kind of important plant pathogens, which can damage trees and crops and cause serious economic losses. Guangdong is located in the subtropical monsoon climate, the climate is warm and humid. In order to identify the species of Rhizoma, and to provide a theoretical basis for the prevention and control of Rhizoma fungi in Guangzhou, this paper investigated and isolated the fungi from Guangzhou and its surrounding areas. The species identification and phylogenetic relationship of the isolated strains were studied by morphological and molecular biological methods. The main results were as follows: a total of 16 strains were isolated from plants in Guangzhou. On the basis of observation of morphological characteristics under standard culture conditions, actin (ACT), calmodulin (CAL), histone H3 (HIS3) was combined with actin. Phylogenetic analysis of 5 gene sequences of translation extension factor (TEF-1 伪) and 尾 -tubulin (TUB2), 16 strains were identified as Ca.spathiphyllicola, in 11 species of: Calonectria cylindrospora complex of 5 complex groups. Red thousand layer red shell Ca.callistemonii, star red shell Ca.spathiphylli, Hodgsly red shell Ca.hodgesii; The Red Shell Ca.crousiana; in the Oriental Red Shell Ca.orientalis;Ca.reteaudii complex of the Ca.brassicae complex Ca.tibouchinae, in the Ca.pentaseptata, small asexual Red Shell Ca.microconidialis;Ca.colhounii complex Hong Kong Red Shell Ca.hongkongensis, in Ca.kyotensis complex Group Ca.ilicicola. Among them, Ca.spathiphyllicola,Ca.callistemonii,Ca.tibouchinae is a new species, Ca.orientalis and Ca.hodgesii are new records of China. Signature tree (Clusia rosea), wild peony (Melastoma sp.) Rosa (Rosa sp.) is a new host of Rhizoma. It is a new host of Ca.hodgesii, a new host of Ca.pentaseptata,Ca.crousiana, a new host of (Melaleuca bracteata), a new host of Ca.pentaseptata, a new host of Ca.microconidialis, (Annona squamosa) of Annona and (Ananas sp.) of pineapple. It is a new host of Ca.ilicicola. All the strains were characterized by colony characteristics, microscopic morphology and morphological characteristics. It was found that there was abundant diversity in the morphology of 16 strains of the genus Ceratopsis, and it was difficult to distinguish their species correctly depending on their morphological characteristics. In this study, by combining morphological analysis with ACT,HIS3,TEF-1 伪, CAL and TUB2 gene sequence phylogenetic tree analysis, 16 strains can be effectively identified to the level of the species. ACT gene had the weakest ability to identify the species of Lycidium, while TEF-1 伪 gene was the most effective for the classification of similar species in the complex group.
【学位授予单位】：仲恺农业工程学院
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S432.44
本文编号：2341686
[Abstract]:(Calonectria) fungi are a kind of important plant pathogens, which can damage trees and crops and cause serious economic losses. Guangdong is located in the subtropical monsoon climate, the climate is warm and humid. In order to identify the species of Rhizoma, and to provide a theoretical basis for the prevention and control of Rhizoma fungi in Guangzhou, this paper investigated and isolated the fungi from Guangzhou and its surrounding areas. The species identification and phylogenetic relationship of the isolated strains were studied by morphological and molecular biological methods. The main results were as follows: a total of 16 strains were isolated from plants in Guangzhou. On the basis of observation of morphological characteristics under standard culture conditions, actin (ACT), calmodulin (CAL), histone H3 (HIS3) was combined with actin. Phylogenetic analysis of 5 gene sequences of translation extension factor (TEF-1 伪) and 尾 -tubulin (TUB2), 16 strains were identified as Ca.spathiphyllicola, in 11 species of: Calonectria cylindrospora complex of 5 complex groups. Red thousand layer red shell Ca.callistemonii, star red shell Ca.spathiphylli, Hodgsly red shell Ca.hodgesii; The Red Shell Ca.crousiana; in the Oriental Red Shell Ca.orientalis;Ca.reteaudii complex of the Ca.brassicae complex Ca.tibouchinae, in the Ca.pentaseptata, small asexual Red Shell Ca.microconidialis;Ca.colhounii complex Hong Kong Red Shell Ca.hongkongensis, in Ca.kyotensis complex Group Ca.ilicicola. Among them, Ca.spathiphyllicola,Ca.callistemonii,Ca.tibouchinae is a new species, Ca.orientalis and Ca.hodgesii are new records of China. Signature tree (Clusia rosea), wild peony (Melastoma sp.) Rosa (Rosa sp.) is a new host of Rhizoma. It is a new host of Ca.hodgesii, a new host of Ca.pentaseptata,Ca.crousiana, a new host of (Melaleuca bracteata), a new host of Ca.pentaseptata, a new host of Ca.microconidialis, (Annona squamosa) of Annona and (Ananas sp.) of pineapple. It is a new host of Ca.ilicicola. All the strains were characterized by colony characteristics, microscopic morphology and morphological characteristics. It was found that there was abundant diversity in the morphology of 16 strains of the genus Ceratopsis, and it was difficult to distinguish their species correctly depending on their morphological characteristics. In this study, by combining morphological analysis with ACT,HIS3,TEF-1 伪, CAL and TUB2 gene sequence phylogenetic tree analysis, 16 strains can be effectively identified to the level of the species. ACT gene had the weakest ability to identify the species of Lycidium, while TEF-1 伪 gene was the most effective for the classification of similar species in the complex group.
【学位授予单位】：仲恺农业工程学院
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S432.44
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1 殷瑜;广州地区丽赤壳属真菌的种类鉴定[D];仲恺农业工程学院;2016年
，本文编号：2341686
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