三峡库区城乡消落带人工植被恢复土壤放线菌多样性特征

发布时间：2018-11-23 10:10

【摘要】：为研究人工植被恢复后消落带土壤放线菌群落多样性特征,以三峡库区典型城市消落带重庆江北嘴大剧院消落带和乡村消落带忠县汝溪河流域消落带为研究区域,于2015年6月原位采集0~20 cm、20~40 cm、40~60 cm土壤,利用限制性末端长度多态性(terminal restriction fragment length polymorphism,T-RFLP)方法,对消落带人工恢复植被土壤不同土层的放线菌群落多样性进行研究.结果发现:(1)三峡库区城乡消落带人工植被恢复后的土壤放线菌多样性有差异,表现为城市消落带土壤放线菌多样性高于乡村消落带土壤(P0.05),特别是在表层土壤.(2)在城乡消落带中,林地土壤放线菌多样性高于草地,且在土层之间放线菌多样性无显著性差异.(3)三峡库区城乡消落带人工植被恢复后的土壤放线菌优势种有差异.(4)冗余分析(redundancy analysis,RDA)和蒙特卡罗置换检验(Monte-Carlo permutation test)表明,城市消落带中含水量、速效氮含量显著影响放线菌群落多样性;乡村消落带中土壤含水量、有机质、速效氮和速效钾含量显著影响放线菌群落多样性.结论表明在消落带人工植被修复后,城市消落带土壤放线菌多样性均高于乡村消落带,这有可能是人为干扰和土壤养分增加所致.
[Abstract]:In order to study the diversity of soil actinomycetes community in the declination zone after the restoration of artificial vegetation, the typical urban decaying zone of the three Gorges Reservoir area, Chongqing Jiangbeizi Grand Theater, and the Zhongnian Ruxi River Basin, were used as the study areas. The diversity of actinomycetes community in different soil layers of different soil layers was studied by using restriction end length polymorphism (terminal restriction fragment length polymorphism,T-RFLP) method in 20 cm,20~40 cm,40~60 cm soil samples collected in June 2015. The results showed that: (1) the diversity of soil actinomycetes in the urban and rural areas of the three Gorges Reservoir area was higher than that in the rural areas (P0.05), and the diversity of soil actinomycetes in the urban declination zone was higher than that in the rural areas (P0.05). Especially in the surface soil. (2) the diversity of actinomycetes in woodland is higher than that in grassland in urban and rural areas. There was no significant difference in actinomycetes diversity among soil layers. (3) the dominant species of soil actinomycetes were different after the restoration of artificial vegetation in urban and rural areas of the three Gorges Reservoir area. (4) redundant analysis of (redundancy analysis,. RDA) and Monte Carlo replacement test (Monte-Carlo permutation test) showed that the water content and available nitrogen content in the urban decaying zone significantly affected the diversity of actinomycetes community. The soil moisture content, organic matter, available nitrogen and available potassium contents significantly affected the diversity of actinomycetes community. The results showed that the diversity of soil actinomycetes in the urban belt was higher than that in the rural zone after the restoration of artificial vegetation, which might be caused by human disturbance and the increase of soil nutrients.
【作者单位】：西南大学生命科学学院三峡库区生态环境教育部重点实验室;
【基金】：重庆市林业重点科技攻关项目(渝林科研2016-8,渝林科研2015-6) 国家国际科技合作专项(2015DFA90900) 中央财政林业科技推广示范项目(渝林科推[2014-10]) 重庆市研究生科研创新项目(CYS2015068)
【分类号】：S154.3;X171.4

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