Act0988拮抗菌株鉴定及培养条件与菌株生长关系研究
发布时间：2018-11-23 11:17
【摘要】：Act0988菌株产对柑橘青霉等多种霉菌具抗菌活性的代谢产物,对菌株进行鉴定、研究培养条件与菌株生长的关系,以为后续开发菌株产抗菌物质的培养基筛选及发酵工艺技术的研究奠定基础。以菌株的形态、培养特征、生理生化特征及16S rDNA序列分析进行菌种鉴定,三角瓶液体振荡培养研究碳氮源、温度、pH及氧与菌株生长的关系。结果表明,Act0988菌株为多产色链霉菌(Streptomyces polychromogenes),菌株生长最佳碳源为可溶性淀粉,发酵液生物量5.8 mg/mL;酵母膏与蛋白胨为最佳氮源,生物量5.7 mg/mL左右。菌株最适温度28℃,生物量6.0 mg/mL。最适初始pH7.0,生物量6.2 mg/mL;最适装液量60 mL/500mL,生物量6.3 mg/mL。Act0988菌株易培养,发酵液抗菌活性强,菌株产抗菌物质具有突出的开发利用潜力。
[Abstract]:Act0988 strains produced metabolites with antibacterial activity against many fungi, such as Penicillium Citrus. The strains were identified, and the relationship between the culture conditions and the growth of the strains was studied. It lays a foundation for the further development of the culture medium for the production of antimicrobial substances and the study of fermentation technology. The strain morphology, culture characteristics, physiological and biochemical characteristics and 16s rDNA sequence analysis were used to identify the strain. The relationship between carbon and nitrogen source, temperature, pH and oxygen and the growth of the strain was studied by liquid oscillatory culture in triangular flask. The results showed that the best carbon source for the growth of Streptomyces polychromostreptomyces (Streptomyces polychromogenes), was soluble starch, the biomass of fermentation broth was 5.8 mg/mL; yeast extract and peptone was the best nitrogen source, and the biomass was about 5.7 mg/mL. Strain temperature 28 鈩，
本文编号：2351397
[Abstract]:Act0988 strains produced metabolites with antibacterial activity against many fungi, such as Penicillium Citrus. The strains were identified, and the relationship between the culture conditions and the growth of the strains was studied. It lays a foundation for the further development of the culture medium for the production of antimicrobial substances and the study of fermentation technology. The strain morphology, culture characteristics, physiological and biochemical characteristics and 16s rDNA sequence analysis were used to identify the strain. The relationship between carbon and nitrogen source, temperature, pH and oxygen and the growth of the strain was studied by liquid oscillatory culture in triangular flask. The results showed that the best carbon source for the growth of Streptomyces polychromostreptomyces (Streptomyces polychromogenes), was soluble starch, the biomass of fermentation broth was 5.8 mg/mL; yeast extract and peptone was the best nitrogen source, and the biomass was about 5.7 mg/mL. Strain temperature 28 鈩，
本文编号：2351397
本文链接：https://www.wllwen.com/kejilunwen/nykj/2351397.html
