水分条件对滨海芦苇湿地土壤微生物多样性的影响

发布时间：2018-11-25 12:11

【摘要】：滨海湿地因受河流和海水的交互作用,其土壤氧化还原电位及营养元素等条件复杂且处于不断变化之中,为了阐明此种环境下不同水分条件对滨海芦苇湿地土壤微生物多样性的影响,尤其是对铁还原菌群落结构的影响,作者以黄河三角洲滨海湿地为对象,研究了淹水和非淹水芦苇湿地表层(0~30 cm)土壤理化特性、微生物多样性(采用T-RFLP技术)和培养条件下湿地土壤微生物的Fe(III)还原能力的变化。结果表明,淹水芦苇湿地土壤有机碳、全氮、盐度、Fe(II)含量和pH值显著(P0.05)高于非淹水芦苇湿地土壤,而淹水芦苇湿地土壤铵态氮含量显著(P0.05)低于非淹水芦苇湿地。淹水芦苇湿地土壤主要优势细菌和古菌分别为铬还原菌属(Alishewanella)、纤维单胞菌属(Cellulomonas)、鞘脂单胞菌属(Sphingomonas)、假单胞菌属(Pseudomonas)和甲烷杆菌属(Methanobacterium)、甲烷八叠球菌属(Methanosarcina);而纤维单胞菌属、食酸菌属(Acidovorax)、地杆菌属(Geobacter)、硫杆菌属(Thiobacillus)和甲烷八叠球菌属分别为非淹水芦苇湿地土壤主要优势细菌和古菌。淹水芦苇湿地土壤主要优势铁还原菌为副球菌属(Paracoccus)、地杆菌属、铬还原菌属和硫单胞菌属(Thiomonas),而非淹水芦苇湿地土壤为地杆菌属和固氮螺菌属(Azospira)。说明淹水能增加芦苇湿地土壤细菌、古菌和铁还原菌的多样性。培养条件下,淹水芦苇湿地土壤铁还原菌的还原Fe(III)能力在培养后期(25d后)低于非淹水芦苇湿地,说明淹水条件在一定程度上抑制了芦苇湿地土壤铁还原菌的铁还原能力。
[Abstract]:Due to the interaction between rivers and seawater, the soil redox potential and nutrient elements in coastal wetland are complex and changing. In order to elucidate the effects of different water conditions on soil microbial diversity in coastal Reed wetland, especially on the structure of ferric reducing bacteria community, the authors take coastal wetland in the Yellow River Delta as an object. The soil physicochemical properties, microbial diversity (T-RFLP technique) and Fe (III) reduction ability of soil microbes in the surface layer (0 ~ 30 cm) of submerged and non-flooded Reed wetland were studied in this paper. The results showed that the soil organic carbon, total nitrogen, Fe (II) content and pH value of submerged Reed wetland were significantly higher than those of non-flooded Reed wetland soil (P0.05). The content of ammonium nitrogen in submerged Reed wetland was significantly lower than that in non-flooded Reed wetland (P0.05). The main dominant bacteria and archaea in flooded Reed wetland soil are chromium-reducing bacteria, (Alishewanella), fiberomonas, (Cellulomonas), sphingomonas, (Sphingomonas), pseudomonas, (Pseudomonas) and methanobacterium (Methanobacterium), respectively. (Methanosarcina); The main dominant bacteria and ancient bacteria in unflooded Reed wetland soil were Cellomonas, (Acidovorax), (Geobacter), Thiobacillus, (Thiobacillus) and Methanobacillus, respectively. The dominant ferric reducing bacteria in submerged Reed wetland soil are (Paracoccus), chromium reducing bacteria and thiomonas (Thiomonas), and non-flooded Reed wetland soil are Dibacillus and (Azospira). The results showed that flooding could increase the diversity of bacteria, archaea and iron reducing bacteria in Reed wetland soil. Under the culture condition, the ability of reducing Fe (III) of ferric reducing bacteria in submerged Reed wetland was lower than that in non-flooded Reed wetland at the later stage of culture (25 days later). The results showed that the inundation condition inhibited the iron reducing ability of soil iron reducing bacteria in Reed wetland to some extent.
【作者单位】：中国科学院烟台海岸带研究所海岸带生物学与生物资源利用所重点实验室;中国科学院大学;滨州医学院;
【基金】：国家自然科学基金面上项目(41371257;41573071);国家自然科学基金青年项目(41401285;31600370) 中国科学院重点部署项目(KZZD-EW-14);中国科学院前沿科学重点研究项目(QYZDJ-SSW-DQC015) 泰山学者青年专家项目(tsqn20161054)~~
【分类号】：S154.3

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