典型岩溶流域不同生态环境土壤细菌多样性特征研究
发布时间：2018-12-07 14:29
【摘要】：全球环境变化和碳循环研究受到关注,岩溶作用具有碳汇效应。为了弄清土壤微生物在岩溶碳循环及碳汇中的作用,有必要对岩溶流域土壤微生物多样性特征进行研究,但目前这方面的研究比较缺乏。本文以长江流域、乌江流域上游段和乌江鸭池河小流域为例,采用PCR-DGGE技术研究了典型岩溶流域不同生态环境下表层土壤细菌多样性的时空特征,初步分析了典型岩溶流域不同生态环境下土壤细菌多样性与土壤基本理化性质及土壤CA活性之间的关系,并初步探讨了典型岩溶流域土壤细菌多样性对岩溶碳循环的指示意义,为深入研究岩溶流域生态环境对微生物多样性及其介导的岩溶碳汇作用的影响奠定了基础。本文获得的主要结果如下:(1)长江流域表层土壤细菌群落多样性指数和丰富度受样地植被类型影响,并表现出明显的季节变化特征,即多样性指数和丰富度均表现为冬季秋季夏季春季(P0.05)。相关分析表明,细菌群落多样性指数和丰富度与土壤速效氮、速效磷和速效钾均呈显著正相关性(P0.01),与SOC和土壤CA活性亦呈一定程度的正相关。(2)乌江流域上游段不同土地利用方式下表层土壤细菌群落多样性指数和丰富度存在差异,平均多样性指数和丰富度表现为乔木林地灌丛农业用地。乌江鸭池河小流域土地利用方式对表层土壤细菌群落样性指数和丰富度影响不明显。相关分析表明,乌江流域上游段表层土壤细菌群落多样性指数和丰富度均与SOC呈显著正相关(P0.01),而且还与土壤含水率、土壤速效氮、速效磷和土壤CA活性呈正相关。乌江鸭池河小流域土壤细菌群落多样性指数和丰富度与土壤CA活性呈一定程度的正相关。综上所述,典型岩溶流域表层土壤细菌群落多样性指数和丰富度受到样地生态环境的影响,且与土壤CA活性和SOC基本呈一定程度的正相关,暗示岩溶流域土壤细菌在岩溶碳循环及土壤固碳中可能扮演了积极的角色,其在流域岩溶生态系统土壤中的固碳作用强度值得深入研究。
[Abstract]:The study of global environmental change and carbon cycle has attracted much attention, and karstification has the effect of carbon sink. In order to understand the role of soil microorganisms in karst carbon cycle and carbon sink, it is necessary to study the characteristics of soil microbial diversity in karst watershed. Taking the Yangtze River Basin, the Upper reaches of the Wujiang River Basin and the small Watershed of the Aechi River of Wujiang River as examples, the temporal and spatial characteristics of bacterial diversity in surface soil of typical karst watershed under different ecological environments were studied by PCR-DGGE technique. The relationship between soil bacterial diversity, soil physical and chemical properties and soil CA activity in different ecological environments in typical karst watershed was analyzed, and the significance of soil bacterial diversity to karst carbon cycle in typical karst watershed was discussed. It lays a foundation for further study on the influence of ecological environment on microbial diversity and karst carbon sink in karst watershed. The main results obtained in this paper are as follows: (1) the diversity index and richness of bacterial communities in the surface soil of the Yangtze River Basin are affected by the vegetation types of the sample plots, and show obvious seasonal variation characteristics. In other words, the diversity index and richness showed winter, autumn, summer and spring (P0.05). Correlation analysis showed that the diversity index and richness of bacterial community were positively correlated with soil available nitrogen, available phosphorus and available potassium (P0.01). There was also a positive correlation with the activities of SOC and soil CA. (2) the diversity index and richness of surface soil bacterial community were different under different land use patterns in the upper reaches of Wujiang River Basin. The average diversity index and richness were the agricultural land of Arbor woodland and shrub. The effect of land use patterns on the similarity index and richness of bacterial community in surface soil was not obvious in the small watershed of Yazhi River in Wujiang River. Correlation analysis showed that the diversity index and richness of soil bacterial communities in the upper reaches of Wujiang River Basin were positively correlated with SOC (P0.01), but also positively correlated with soil moisture content, soil available nitrogen, available phosphorus and soil CA activity. The diversity index and richness of soil bacterial community were positively correlated with soil CA activity. To sum up, the diversity index and richness of bacterial community in surface soil of typical karst watershed were affected by the ecological environment of sample plots, and there was a positive correlation with soil CA activity and SOC to some extent. It is suggested that soil bacteria may play an active role in karst carbon cycle and soil carbon sequestration in karst watershed, and the intensity of carbon sequestration in karst ecosystem soil is worthy of further study.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S154.36
本文编号：2367344
[Abstract]:The study of global environmental change and carbon cycle has attracted much attention, and karstification has the effect of carbon sink. In order to understand the role of soil microorganisms in karst carbon cycle and carbon sink, it is necessary to study the characteristics of soil microbial diversity in karst watershed. Taking the Yangtze River Basin, the Upper reaches of the Wujiang River Basin and the small Watershed of the Aechi River of Wujiang River as examples, the temporal and spatial characteristics of bacterial diversity in surface soil of typical karst watershed under different ecological environments were studied by PCR-DGGE technique. The relationship between soil bacterial diversity, soil physical and chemical properties and soil CA activity in different ecological environments in typical karst watershed was analyzed, and the significance of soil bacterial diversity to karst carbon cycle in typical karst watershed was discussed. It lays a foundation for further study on the influence of ecological environment on microbial diversity and karst carbon sink in karst watershed. The main results obtained in this paper are as follows: (1) the diversity index and richness of bacterial communities in the surface soil of the Yangtze River Basin are affected by the vegetation types of the sample plots, and show obvious seasonal variation characteristics. In other words, the diversity index and richness showed winter, autumn, summer and spring (P0.05). Correlation analysis showed that the diversity index and richness of bacterial community were positively correlated with soil available nitrogen, available phosphorus and available potassium (P0.01). There was also a positive correlation with the activities of SOC and soil CA. (2) the diversity index and richness of surface soil bacterial community were different under different land use patterns in the upper reaches of Wujiang River Basin. The average diversity index and richness were the agricultural land of Arbor woodland and shrub. The effect of land use patterns on the similarity index and richness of bacterial community in surface soil was not obvious in the small watershed of Yazhi River in Wujiang River. Correlation analysis showed that the diversity index and richness of soil bacterial communities in the upper reaches of Wujiang River Basin were positively correlated with SOC (P0.01), but also positively correlated with soil moisture content, soil available nitrogen, available phosphorus and soil CA activity. The diversity index and richness of soil bacterial community were positively correlated with soil CA activity. To sum up, the diversity index and richness of bacterial community in surface soil of typical karst watershed were affected by the ecological environment of sample plots, and there was a positive correlation with soil CA activity and SOC to some extent. It is suggested that soil bacteria may play an active role in karst carbon cycle and soil carbon sequestration in karst watershed, and the intensity of carbon sequestration in karst ecosystem soil is worthy of further study.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S154.36
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