玫瑰黄链霉菌Men-myco-93-63产抗生素发酵培养基的优化

发布时间：2018-12-14 23:52

【摘要】：以玫瑰黄链霉菌Men-myco-93-63为发酵菌株,采用Plackett-Burman试验设计、最陡爬坡试验、中心复合试验设计和响应面分析法,以抗生素产量为响应值,对其发酵培养基进行优化。首先,采用Plackett-Burman设计筛选出具有正显著效应的花生饼粉和玉米浆。其次,通过最陡爬坡试验逼近最大响应区域。最后,采用中心复合试验对这2个因素进行优化。最终确定发酵的最适培养基含量配方为葡萄糖2%,可溶性淀粉1%,花生饼粉4.96%,玉米浆2.66%,氯化钠0.4%,碳酸钙0.3%,磷酸二氢钾0.02%。优化后发酵培养基抗生素产量为2 030.6 mg/L,比优化前的771.9 mg/L提高了1.63倍。
[Abstract]:Using Streptomyces roseus Men-myco-93-63 as fermentation strain, the fermentation medium was optimized by Plackett-Burman test design, steepest climbing test, central complex test design and response surface analysis. Firstly, peanut cake powder and corn pulp with positive effect were selected by Plackett-Burman design. Secondly, the maximum response region is approximated by the steepest climbing test. Finally, the central composite test was used to optimize the two factors. The optimum medium for fermentation was determined as glucose 2, soluble starch 1, peanut cake powder 4.96, corn pulp 2.66, sodium chloride 0.4, calcium carbonate 0.33, potassium dihydrogen phosphate 0.02. The antibiotic yield of the optimized fermentation medium was 2 030.6 mg/L, which was 1.63 times higher than that of 771.9 mg/L before optimization.
【作者单位】：河北农业大学植物保护学院/河北省农作物病虫害生物防治工程技术研究中心/国家北方山区农业工程技术研究中心;
【基金】：国家自然科学基金(编号:31572050) 河北省中药材产业技术体系(编号:1004029)
【分类号】：S476.1

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