施氮肥对华北平原土壤氨氧化细菌和古菌数量及群落结构的影响
发布时间：2018-12-19 20:50
【摘要】：利用荧光定量PCR、末端限制性片段长度多样性(T-RFLP)和基因克隆文库技术,比较了4种施氮水平(不施氮肥,0 kg N/hm~2,CK;施低水平氮肥,75 kg N/hm~2,N1;施中水平氮肥,150 kg N/hm~2,N2;施高水平氮肥,225 kg N/hm~2,N3)下华北平原地区小麦季表层(0—20 cm)土壤总细菌、氨氧化细菌(AOB)和氨氧化古菌(AOA)的丰度和群落结构。结果表明,土壤总细菌、AOB和AOA数量分别在每克干土5.74×10~9—7.50×10~9、8.89×10~6—2.66×10~7和3.83×10~8—7.78×10~8之间。不同施氮量土壤AOA数量均高于AOB数量,AOA/AOB值在81.72—14.38之间。增施氮肥显著显著提高AOB数量(P0.05),对总细菌和AOA数量的影响不显著(P0.05)。与CK相比,处理N1、N2和N3中AOB数量分别提高了0.64、1.50和1.99倍。增施氮肥显著改变了AOB和AOA的群落结构,且不同施氮量处理中AOB群落结构差异更大。系统进化分析显示,施氮肥小麦土壤AOB主要为Nitrosospira属类群,分布在Cluster 3的两个分支中;AOA分布在Cluster S的4个分支中。相关性分析显示,AOB数量与全氮和铵态氮含量呈显著正相关关系,与土壤pH和碳氮比呈显著负相关关系(P0.05);AOA数量与硝态氮含量和土壤pH呈显著正相关关系,与铵态氮含量呈显著负相关关系(P0.05)。研究结果表明:增施氮肥可显著改变华北平原地区碱性土壤AOB数量与群落结构,该地区小麦土壤中AOB比AOA对氮肥响应更敏感。
[Abstract]:Using fluorescent quantitative PCR, terminal restriction fragment length diversity (T-RFLP) and gene cloning library technique, we compared four nitrogen levels (0 kg N / hmm2 CK, 75 kg N / hmm2 N 1) and 75 kg N / hm2 N 1, respectively. Applying medium level nitrogen fertilizer, 150 kg N / hm ~ (2) N _ (2); The abundance and community structure of soil total bacteria, ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing palaeomycetes (AOA) in wheat season surface layer (0-20 cm) in North China Plain were studied by applying high level nitrogen fertilizer, 225 kg N / hmm-2 N 3). The results showed that the total number of bacteria, AOB and AOA in soil ranged from 5.74 脳 10 ~ (-9) to 7.50 脳 10 ~ (10) ~ 9 ~ (9) C ~ (-1) 8.89 脳 10 ~ (6) ~ 2.66 脳 10 ~ (7) and 3.83 脳 10 ~ (8) 脳 10 ~ (8) 脳 10 ~ (8), respectively. The amount of AOA was higher than that of AOB, and the AOA/AOB value was between 81.72-14.38. Increasing nitrogen fertilizer significantly increased the number of AOB (P0.05), but had no significant effect on total bacteria and AOA (P0.05). Compared with CK, the number of AOB in N _ 1N _ 2 and N _ 3 was increased by 0.64% and 1.99 times, respectively. The community structure of AOB and AOA was significantly changed by nitrogen fertilizer application, and the difference of AOB community structure was greater in different nitrogen application treatments. Phylogenetic analysis showed that the soil AOB of nitrogen fertilized wheat was mainly Nitrosospira group, distributed in two branches of Cluster 3, and AOA was distributed in four branches of Cluster S. Correlation analysis showed that the amount of AOB was positively correlated with total nitrogen and ammonium nitrogen content, and negatively correlated with soil pH and C / N ratio (P0.05). There was a significant positive correlation between the amount of AOA and nitrate nitrogen content and soil pH content, and a significant negative correlation between the amount of AOA and ammonium nitrogen content (P0.05). The results showed that the amount of AOB and community structure of alkaline soil in North China Plain could be significantly changed by increasing nitrogen fertilizer. AOB in wheat soil was more sensitive to nitrogen fertilizer than AOA.
【作者单位】： 中国农业大学农学院;
【基金】：公益性行业(农业)科研专项(201503121-11) 河北省科技攻关项目(14227008D)
【分类号】：S154.3
本文编号：2387434
[Abstract]:Using fluorescent quantitative PCR, terminal restriction fragment length diversity (T-RFLP) and gene cloning library technique, we compared four nitrogen levels (0 kg N / hmm2 CK, 75 kg N / hmm2 N 1) and 75 kg N / hm2 N 1, respectively. Applying medium level nitrogen fertilizer, 150 kg N / hm ~ (2) N _ (2); The abundance and community structure of soil total bacteria, ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing palaeomycetes (AOA) in wheat season surface layer (0-20 cm) in North China Plain were studied by applying high level nitrogen fertilizer, 225 kg N / hmm-2 N 3). The results showed that the total number of bacteria, AOB and AOA in soil ranged from 5.74 脳 10 ~ (-9) to 7.50 脳 10 ~ (10) ~ 9 ~ (9) C ~ (-1) 8.89 脳 10 ~ (6) ~ 2.66 脳 10 ~ (7) and 3.83 脳 10 ~ (8) 脳 10 ~ (8) 脳 10 ~ (8), respectively. The amount of AOA was higher than that of AOB, and the AOA/AOB value was between 81.72-14.38. Increasing nitrogen fertilizer significantly increased the number of AOB (P0.05), but had no significant effect on total bacteria and AOA (P0.05). Compared with CK, the number of AOB in N _ 1N _ 2 and N _ 3 was increased by 0.64% and 1.99 times, respectively. The community structure of AOB and AOA was significantly changed by nitrogen fertilizer application, and the difference of AOB community structure was greater in different nitrogen application treatments. Phylogenetic analysis showed that the soil AOB of nitrogen fertilized wheat was mainly Nitrosospira group, distributed in two branches of Cluster 3, and AOA was distributed in four branches of Cluster S. Correlation analysis showed that the amount of AOB was positively correlated with total nitrogen and ammonium nitrogen content, and negatively correlated with soil pH and C / N ratio (P0.05). There was a significant positive correlation between the amount of AOA and nitrate nitrogen content and soil pH content, and a significant negative correlation between the amount of AOA and ammonium nitrogen content (P0.05). The results showed that the amount of AOB and community structure of alkaline soil in North China Plain could be significantly changed by increasing nitrogen fertilizer. AOB in wheat soil was more sensitive to nitrogen fertilizer than AOA.
【作者单位】： 中国农业大学农学院;
【基金】：公益性行业(农业)科研专项(201503121-11) 河北省科技攻关项目(14227008D)
【分类号】：S154.3
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