保水剂对侧柏容器苗根际土壤微生物种群结构及干旱适应能力的影响
发布时间：2018-12-19 20:56
【摘要】：为更好地将保水剂应用到干旱山地的容器苗造林中,以丙烯酰胺-丙烯酸钾交联共聚物型保水剂作为供试材料,设置育苗时(NS)和造林时(AS)各加4.0 g保水剂的两种保水剂应用方式,利用传统的平板计数法和现代的末端限制性片段长度多态性分析(Terminal restricti on fragment length polymorphism,T-RFLP)技术,研究NS和AS对侧柏容器苗造林一年后根际土壤微生物种群结构及对侧柏干旱适应能力的影响.结果表明:保水剂可显著提高侧柏根际土壤细菌、真菌的数量和微生物量碳、氮的含量,且NS处理根际土壤的细菌数量和微生物量碳分别比AS处理提高21.65%和8.04%;保水剂及其应用方式对造林初期侧柏根际土壤的细菌群落结构产生较大影响,保水剂显著增加了根际土壤中细菌群落结构的多样性,NS处理具有最高的丰富度指数、多样性指数和最低的优势度指数;此外,同对照相比,NS处理还显著提高了侧柏容器苗的造林成活率、叶片的净光合速率和相对含水量,降低了相对电导率;同AS处理相比,NS处理的造林成活率和叶片相对含水量分别提高了11.64%和8.33%,而相对电导率降低(降低12.19%).综上说明,保水剂通过改善侧柏容器苗根际土壤的微生物种群结构而提高植物的干旱适应能力,利用添加保水剂的容器苗造林具有更好的应用效果.
[Abstract]:In order to better apply water-retaining agent to container plantlet afforestation in arid mountain area, acrylamide potassium acrylate crosslinked copolymer type water-retaining agent was used as test material. (Terminal restricti on fragment length polymorphism, was analyzed by using traditional plate counting method and modern terminal restriction fragment length polymorphism (TRFLP) by setting up two water retaining agents, (NS) during seedling raising and (AS) with 4.0g water retaining agent respectively during silviculture, and by means of traditional plate counting method and modern terminal restriction fragment length polymorphism (TRFLP). The effects of NS and AS on the soil microbial population structure and drought adaptability of Platycladus orientalis were studied by T-RFLP technique. The results showed that the amount of bacteria, fungi, microbial biomass carbon and nitrogen in rhizosphere soil of Platycladus orientalis could be increased by 21.65% and 8.04%, respectively, compared with that of AS treatment, and the amount of bacteria and microbial biomass carbon in rhizosphere soil treated with NS were increased by 21.65% and 8.04%, respectively. The microbial community structure of rhizosphere soil of Platycladus orientalis was greatly affected by water retaining agent and its application methods, and the diversity of bacterial community structure in rhizosphere soil was significantly increased by water retaining agent. NS treatment had the highest richness index. Diversity index and lowest dominance index; In addition, compared with the control, NS treatment also significantly increased the survival rate of plantlets, the net photosynthetic rate and relative water content of leaves, and decreased the relative electrical conductivity. Compared with AS treatment, the survival rate and leaf relative water content of NS treatment increased by 11.64% and 8.33%, respectively, while the relative conductivity decreased (12.19%). It is concluded that the water-retaining agent can improve the drought adaptability of the plants by improving the microbial population structure of the rhizosphere soil of the container seedlings of Platycladus orientalis.
【作者单位】： 山东省林业科学研究院山东省森林植被生态修复工程技术研究中心;德州学院;
【基金】：山东省科技发展计划项目(2010GSF10621) 山东省农业重大应用技术创新项目资助~~
【分类号】：S791.38;S714.3
，
本文编号：2387440
[Abstract]:In order to better apply water-retaining agent to container plantlet afforestation in arid mountain area, acrylamide potassium acrylate crosslinked copolymer type water-retaining agent was used as test material. (Terminal restricti on fragment length polymorphism, was analyzed by using traditional plate counting method and modern terminal restriction fragment length polymorphism (TRFLP) by setting up two water retaining agents, (NS) during seedling raising and (AS) with 4.0g water retaining agent respectively during silviculture, and by means of traditional plate counting method and modern terminal restriction fragment length polymorphism (TRFLP). The effects of NS and AS on the soil microbial population structure and drought adaptability of Platycladus orientalis were studied by T-RFLP technique. The results showed that the amount of bacteria, fungi, microbial biomass carbon and nitrogen in rhizosphere soil of Platycladus orientalis could be increased by 21.65% and 8.04%, respectively, compared with that of AS treatment, and the amount of bacteria and microbial biomass carbon in rhizosphere soil treated with NS were increased by 21.65% and 8.04%, respectively. The microbial community structure of rhizosphere soil of Platycladus orientalis was greatly affected by water retaining agent and its application methods, and the diversity of bacterial community structure in rhizosphere soil was significantly increased by water retaining agent. NS treatment had the highest richness index. Diversity index and lowest dominance index; In addition, compared with the control, NS treatment also significantly increased the survival rate of plantlets, the net photosynthetic rate and relative water content of leaves, and decreased the relative electrical conductivity. Compared with AS treatment, the survival rate and leaf relative water content of NS treatment increased by 11.64% and 8.33%, respectively, while the relative conductivity decreased (12.19%). It is concluded that the water-retaining agent can improve the drought adaptability of the plants by improving the microbial population structure of the rhizosphere soil of the container seedlings of Platycladus orientalis.
【作者单位】： 山东省林业科学研究院山东省森林植被生态修复工程技术研究中心;德州学院;
【基金】：山东省科技发展计划项目(2010GSF10621) 山东省农业重大应用技术创新项目资助~~
【分类号】：S791.38;S714.3
，
本文编号：2387440
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