不同寄主来源轮枝孢菌的交互致病性

发布时间：2018-12-27 12:39

【摘要】：为了明确不同寄主来源的轮枝孢菌是否存在交互致病性,对来源于向日葵、棉花、茄子、生菜和马铃薯5种不同寄主植物上的10株轮枝孢菌的生物学特性,如菌落形态、生长速度、生理小种、交配型和致病力分化进行研究,并利用大丽轮枝孢菌不同生理小种和交配型的特异引物对供试大丽轮枝孢菌菌株进行聚合酶链式反应(polymerase chain reaction,PCR)检测。结果表明,不同寄主来源的轮枝孢菌在菌落形态和生长速度上存在显著差异。PCR检测结果表明:除了来自生菜的大丽轮枝孢菌Ls16-1为1号生理小种外,其余8株不同寄主来源的大丽轮枝孢菌均为2号生理小种;所有供试大丽轮枝孢菌菌株的交配型均为MAT1-2-1型。此外,采用纸钵撕底蘸根法将不同寄主来源的轮枝孢菌分别接种向日葵、棉花、茄子和马铃薯。结果显示,不同寄主来源的菌株对上述不同寄主均表现出不同程度的致病力,但都对自身分离寄主的致病性表现最强。综上表明,不同寄主来源的轮枝孢菌对不同寄主存在交互致病性,但均对其分离寄主的致病性表现最强。
[Abstract]:In order to find out whether there is cross-pathogenicity in different host species, the biological characteristics, such as colony morphology, of 10 strains of Trichoderma from 5 different host plants, such as sunflower, cotton, eggplant, lettuce and potato, were studied. The growth rate, physiological races, mating type and pathogenicity differentiation were studied. Polymerase chain reaction (polymerase chain reaction,) was used to detect the specific primers of different physiological races and mating types of Trichoderma dahliensis. PCR). The results showed that there were significant differences in colony morphology and growth rate among different host sources. The results of PCR showed that Ls16-1 from lettuce was a physiological race 1. The other 8 strains of Trichoderma dahliensis from different host sources were all physiological races 2; The mating type of all the tested strains of Trichoderma dahliensis was MAT1-2-1. In addition, the root dipping method of paper bowl was used to inoculate different host fungi with sunflower, cotton, eggplant and potato respectively. The results showed that the strains from different host sources showed different pathogenicity to the above hosts, but the pathogenicity of the isolates was the strongest. The results showed that there was mutual pathogenicity of different host strains on different hosts, but the pathogenicity of each of them was the strongest.
【作者单位】：内蒙古农业大学农学院;乌拉特前旗农业技术推广中心;
【基金】：国家向日葵产业技术体系项目(CARS-16) 国家公益性行业(农业)科研专项(201103016)
【分类号】：S432.44

【相似文献】