红毛草入侵程度对根际土壤微生物的影响
发布时间：2018-12-31 15:56
【摘要】：【目的】研究外来种入侵对地下土壤微生物的影响,从植物与地下微生物互作的角度探索外来种的入侵机制。【方法】通过磷脂脂肪酸(PLFA)分析法和比色法,研究红毛草(Rhynchelytrum repens)不同入侵程度(未入侵、轻度入侵和中度入侵)对根际土壤理化性质、微生物群落结构、特殊功能微生物及其多样性水平的影响。【结果】供试土样中共检测到33种PLFA生物标记,其中细菌24种、放线菌4种、真菌4种、原生动物1种。随红毛草入侵程度增加,土壤碱解氮、全氮含量显著增加,含水量显著减少,速效磷、速效钾含量和pH无显著变化;根际土壤中细菌、真菌、原生动物以及PLFA标记物总含量均呈现逐渐增加的趋势;革兰氏阳性菌与革兰氏阴性菌含量比值逐渐减小;功能微生物含量逐渐增加,与未入侵地相比,红毛草重度入侵地根际土壤中的丛枝菌根真菌含量增加了42.46%,伯克霍尔德菌含量增加了22.32%,嗜热解氢杆菌增加了13.08%。红毛草入侵对根际土壤中微生物多样性水平有一定影响,Shannon-Wiener多样性和丰富度指数随入侵程度增加呈现升高的趋势,Pielou均匀度指数降低,Simpson优势度指数无显著变化。土壤中细菌含量与土壤全氮、碱解氮含量之间呈显著正相关;真菌含量与土壤碱解氮含量呈显著正相关。【结论】红毛草的入侵改变了根际土壤微生物的群落结构和土壤理化性质。随入侵的加剧,土壤全氮和碱解氮含量增加,细菌、真菌和原生动物含量以及一些特殊功能性微生物含量增多,土壤微生物群落多样性指数和丰富度指数增大。
[Abstract]:[objective] to study the influence of alien species invasion on soil microbes, and to explore the invasion mechanism of alien species from the perspective of the interaction between plants and underground microbes. [methods] the invasion mechanism of alien species was studied by (PLFA) analysis and colorimetric analysis of phospholipid fatty acids. To study the physical and chemical properties and microbial community structure of rhizosphere soil with different degrees of (Rhynchelytrum repens) invasion (non-invasion, mild invasion and moderate invasion). [results] A total of 33 PLFA biomarkers were detected in soil samples, including 24 species of bacteria, 4 species of actinomycetes, 4 species of fungi and 1 species of protozoa. With the increase of invasion degree, soil alkali-hydrolyzed nitrogen, total nitrogen content, water content significantly decreased, available phosphorus, available potassium content and pH did not change significantly. The total contents of bacteria, fungi, protozoa and PLFA markers in rhizosphere soil increased gradually, and the ratio of Gram-positive bacteria to Gram-negative bacteria decreased gradually. The content of arbuscular mycorrhizal fungi increased by 42.46 in the rhizosphere soil, and the content of Bachelia burkholdii increased by 22.32% in the Rhizosphere soil. The number of hydrophilic bacteria increased by 13.08. Shannon-Wiener diversity and richness index increased with the increase of invasion degree, Pielou evenness index decreased, and Simpson dominance index did not change significantly. There was a significant positive correlation between bacterial content and soil total nitrogen and alkali-hydrolyzed nitrogen content. [conclusion] the invasion of Rhizopsis rubrum changed the community structure and physical and chemical properties of soil microbes in the rhizosphere. With the increase of invasion, the contents of total nitrogen and alkali-hydrolyzed nitrogen increased, the contents of bacteria, fungi and protozoa and some special functional microbes increased, and the diversity index and richness index of soil microbial community increased.
【作者单位】： 西北师范大学生命科学学院;韩山师范学院生命科学与食品科技学院;韩山师范学院地理学系;
【基金】：国家自然科学基金项目(31470021) 广东韩山师范学院科研团队项目(LT201204)
【分类号】：S154.3;S45
本文编号：2396805
[Abstract]:[objective] to study the influence of alien species invasion on soil microbes, and to explore the invasion mechanism of alien species from the perspective of the interaction between plants and underground microbes. [methods] the invasion mechanism of alien species was studied by (PLFA) analysis and colorimetric analysis of phospholipid fatty acids. To study the physical and chemical properties and microbial community structure of rhizosphere soil with different degrees of (Rhynchelytrum repens) invasion (non-invasion, mild invasion and moderate invasion). [results] A total of 33 PLFA biomarkers were detected in soil samples, including 24 species of bacteria, 4 species of actinomycetes, 4 species of fungi and 1 species of protozoa. With the increase of invasion degree, soil alkali-hydrolyzed nitrogen, total nitrogen content, water content significantly decreased, available phosphorus, available potassium content and pH did not change significantly. The total contents of bacteria, fungi, protozoa and PLFA markers in rhizosphere soil increased gradually, and the ratio of Gram-positive bacteria to Gram-negative bacteria decreased gradually. The content of arbuscular mycorrhizal fungi increased by 42.46 in the rhizosphere soil, and the content of Bachelia burkholdii increased by 22.32% in the Rhizosphere soil. The number of hydrophilic bacteria increased by 13.08. Shannon-Wiener diversity and richness index increased with the increase of invasion degree, Pielou evenness index decreased, and Simpson dominance index did not change significantly. There was a significant positive correlation between bacterial content and soil total nitrogen and alkali-hydrolyzed nitrogen content. [conclusion] the invasion of Rhizopsis rubrum changed the community structure and physical and chemical properties of soil microbes in the rhizosphere. With the increase of invasion, the contents of total nitrogen and alkali-hydrolyzed nitrogen increased, the contents of bacteria, fungi and protozoa and some special functional microbes increased, and the diversity index and richness index of soil microbial community increased.
【作者单位】： 西北师范大学生命科学学院;韩山师范学院生命科学与食品科技学院;韩山师范学院地理学系;
【基金】：国家自然科学基金项目(31470021) 广东韩山师范学院科研团队项目(LT201204)
【分类号】：S154.3;S45
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1 陈彦;杨中艺;袁剑刚;;红毛草Rhynchelytrum repens(Willd.)C.E.Hubbard的繁殖特性[J];中山大学学报(自然科学版);2013年05期
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