大青山不同树种根围土壤酶活性与微生物群落丰度的研究

发布时间：2019-01-04 06:42

【摘要】：针对大青山山地森林不同树种的根围土壤,探讨微生物丰度与土壤酶活性之间的联系以及受控因子。利用荧光实时定量PCR方法研究不同树种土壤微生物丰度的变化情况,分析土壤β-葡萄糖苷酶(βG)、N-乙酰氨基葡萄糖苷酶(NAG)和过氧化物酶(Pod)活性以及土壤理化性质的变化趋势。采用主成分分析(PCA)和皮尔森相关性分析方法研究土壤理化因子对土壤微生物群落丰度的影响。结果显示,土壤理化性质和微生物细菌、真菌群落丰度从春季到秋季均显著增高,如有机碳、全氮、微生物量碳和氮数量及细菌、真菌丰度等;大青山森林土壤βG、Pod酶活性夏季较高,而NAG酶活性秋季较高。皮尔森相关性分析表明大青山不同树种土壤酶活性与土壤微生物丰度有明显的相关性。与NAG酶活性极显著正相关(P0.05);与Pod酶活性呈极显著负相关(P0.05);而βG只与细菌群落丰度呈极显著负相关(P0.05)。土壤理化因子(有机碳、全氮、微生物量碳氮)与土壤微生物群落丰度均表现为极显著正相关。主成分分析认为,土壤有机碳、全氮、微生物量碳和氮、细菌和真菌群落丰度、N-乙酰氨基葡萄糖苷酶等可作为影响不同树种根围土壤养分特性的重要因子。在大青山山地森林生态系统,不同树种对土壤理化指标、土壤微生物丰度和土壤酶活性影响较大,并且随着季节变化响应较强。理化指标和土壤微生物群落丰度是调控大青山森林植被根围土壤微生物群落的主要生态因子。
[Abstract]:The relationship between microbial abundance and soil enzyme activity and the controlled factors were studied in the soil of different tree species in mountain forest of Daqingshan. The variation of soil microbial abundance of different tree species was studied by real-time fluorescence quantitative PCR, and the 尾 G), in soil was analyzed. The change trend of the activities of N-acetylglucosaminidase (NAG) and peroxidase (Pod) and the physical and chemical properties of soil. The effects of soil physical and chemical factors on the abundance of soil microbial communities were studied by principal component analysis (PCA) and Pearson correlation analysis. The results showed that the physical and chemical properties of soil and the abundance of bacteria and fungi increased significantly from spring to autumn, such as organic carbon, total nitrogen, microbial biomass carbon and nitrogen, bacteria, fungi abundance and so on. The activity of 尾 -Gelopod in forest soil of Daqingshan was higher in summer, while that of NAG in autumn was higher. Pearson correlation analysis showed that soil enzyme activity of different tree species in Daqing Mountain was significantly correlated with soil microbial abundance. There was a very significant positive correlation between 尾 G and NAG activity (P0.05); a very significant negative correlation with Pod activity (P0.05); while 尾 G was negatively correlated with bacterial community abundance (P0.05). Soil physicochemical factors (organic carbon, total nitrogen, microbial biomass carbon) were significantly positively correlated with soil microbial community abundance. Principal component analysis showed that soil organic carbon, total nitrogen, microbial biomass carbon and nitrogen, bacterial and fungal abundance and N-acetyl glucosaminidase could be considered as important factors affecting soil nutrient characteristics in root circumference of different tree species. In the mountain forest ecosystem of Daqingshan, different tree species have great influence on soil physical and chemical index, soil microbial abundance and soil enzyme activity, and have a strong response with seasonal changes. The physical and chemical indexes and the abundance of soil microbial communities were the main ecological factors regulating the soil microbial communities in the root environs of forest vegetation in Daqingshan.
【作者单位】：内蒙古农业大学生命科学学院;内蒙古和盛生态科技研究院有限公司;内蒙古师范大学生命科学与技术学院;内蒙古农业大学林学院;
【基金】：内蒙古自治区高等学校科学研究项目(NJZY14097) 呼和浩特市科技计划项目(重大科技专项)(2015-重-社-1)
【分类号】：S714.3

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