貂粪堆肥微生物复合菌剂的制备及效果评价
发布时间：2019-02-28 15:07
【摘要】：随着经济和社会的迅速发展,我国的养殖业趋于规模化和集约化,随之带来了严重的养殖业污染问题。堆肥能够实现养殖业废弃物的无害化和资源化处理,但是传统的堆肥存在耗时长、发酵不彻底等诸多弊端,因此在堆肥中添加微生物复合菌剂以实现养殖业废弃物的快速堆肥处理具有重要的现实意义。本论文旨在制备用于貂粪堆肥的微生物复合菌剂以实现貂粪的快速堆肥无害化处理。具体的研究内容如下：(1)建立普通好氧堆肥,在堆肥过程中的不同阶段取样,从样品中筛选到堆肥微生物共20余种,经过菌落形态特征方面的比较进行分类合并,最后得到Al,A2,A3,A4, A5, A6, A7, A8共8种堆肥微生物；(2)对筛选的堆肥微生物菌种进行16S rDNA鉴定以及其他生理生化功能鉴定,最后确定分别与Bacillus amyloliquefaciens, Bacillus flexus、Bacillus subtilis, Ureibacillus thermosphaericus, Geobacillus sp., Geobacillus pallidus, Bacillus licheniformis, Ureibacillus sp.具有很高的相似性,并且都具有各自独特的功能,将其扩大培养后辅以载体制成微生物复合菌剂ABTNL Ⅱ备用；(3)将自主研发的微生物复合菌剂接种于貂粪堆肥中,检测物理、化学以及生物学指标并进行比较,结果显示,实验组当天即进入高温期(55℃以上),之后持续14d,最高温度达到64.1℃,显著高于对照组的59℃；而第3d才开始升温的对照组的高温期仅仅持续了7d；实验组第5d已基本上没有臭味,但对照组第7d仍能闻到臭味；貂粪堆肥过程中,添加微生物复合菌剂的实验组的各项化学指标(pH值、C/N、电导率、E4/E6、水溶性氨氮和硝氮)的变化与空白对照组相比均更为明显且更加理想；实验组的发芽指数在第10d即达到80%以上,堆肥结束后达95%以上,而对照组在堆肥结束后仍未能达到80%。综上所述,貂粪堆肥中添加微生物复合菌剂不仅能够促进其快速升温,而且能够大大延长高温持续时间,缩短堆肥周期,加速堆肥中大分子营养物质和植物毒性物质的降解和腐殖化。可以将腐熟产品制成有机肥或生物有机肥,从而有效地保证了貂粪的无害化处理和资源化利用。
[Abstract]:With the rapid development of economy and society, China's aquaculture industry tends to scale and intensive, resulting in serious pollution problems. Compost can realize harmless and resource treatment of aquaculture waste, but traditional compost has many disadvantages such as time-consuming, fermentation incomplete and so on. Therefore, it is of great practical significance to add microbial compound fungicides into compost to realize rapid composting treatment of aquaculture waste. The aim of this paper is to prepare microbial composting agent for mink manure composting to realize the harmless treatment of mink manure composting. The specific research contents are as follows: (1) establishing common aerobic compost, sampling in different stages of composting process, screening out more than 20 kinds of compost microorganism from the sample, and combining the colony morphological characteristics. Finally, eight composting microorganisms, Al,A2,A3,A4, A5, A6, A7 and A8, were obtained. (2) 16s rDNA and other physiological and biochemical functions were used to identify the selected microbial strains of compost. Finally, the strain was identified with Bacillus amyloliquefaciens, Bacillus flexus,Bacillus subtilis, Ureibacillus thermosphaericus, Geobacillus sp., Geobacillus pallidus, Bacillus licheniformis, Ureibacillus sp., respectively. It has very high similarity, and all have their own unique functions. After the expanded culture, the microbial compound agent ABTNL 鈪，
本文编号：2431910
[Abstract]:With the rapid development of economy and society, China's aquaculture industry tends to scale and intensive, resulting in serious pollution problems. Compost can realize harmless and resource treatment of aquaculture waste, but traditional compost has many disadvantages such as time-consuming, fermentation incomplete and so on. Therefore, it is of great practical significance to add microbial compound fungicides into compost to realize rapid composting treatment of aquaculture waste. The aim of this paper is to prepare microbial composting agent for mink manure composting to realize the harmless treatment of mink manure composting. The specific research contents are as follows: (1) establishing common aerobic compost, sampling in different stages of composting process, screening out more than 20 kinds of compost microorganism from the sample, and combining the colony morphological characteristics. Finally, eight composting microorganisms, Al,A2,A3,A4, A5, A6, A7 and A8, were obtained. (2) 16s rDNA and other physiological and biochemical functions were used to identify the selected microbial strains of compost. Finally, the strain was identified with Bacillus amyloliquefaciens, Bacillus flexus,Bacillus subtilis, Ureibacillus thermosphaericus, Geobacillus sp., Geobacillus pallidus, Bacillus licheniformis, Ureibacillus sp., respectively. It has very high similarity, and all have their own unique functions. After the expanded culture, the microbial compound agent ABTNL 鈪，
本文编号：2431910
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