生草对沙地葡萄园土壤微生物群落碳源利用特征的影响

发布时间：2019-03-07 18:06

【摘要】：为了研究葡萄园行间生草处理对土壤微生物群落功能多样性的影响,以清耕为对照,运用BiologEco板技术,分析生草处理下的土壤微生物群落碳源利用特征。结果表明:生草处理的AWCD值和多样性指数(Shannon-Wiener多样性指数、Simpson优势度指数和丰富度指数)均显著高于清耕对照,且随土层深度的增加均呈下降趋势,以生草0~20 cm土层处理最高;与清耕对照相比,生草处理土壤微生物对6类碳源利用强度均显著增加,平均增幅顺序为:羧酸类化合物酚酸类化合物胺类化合物碳水化合物类氨基酸类聚合物。主成份分析表明,清耕对照和生草处理分布在不同区域,表明两处理土壤微生物存在一定的代谢差异性,生草显著改变了土壤微生物群落功能,并对土壤微生物代谢羧酸类化合物、碳水化合物和酚酸类化合物3类碳源的影响最为明显。结果提示,生草有利于葡萄园土壤微生物群落结构的改善。
[Abstract]:In order to study the effect of interrow grass treatment in vineyard on the functional diversity of soil microbial community, the characteristics of carbon source utilization in soil microbial community were analyzed by using Biology Ecoboard technique with clean tillage as control. The results showed that the AWCD value and diversity index (Shannon-Wiener diversity index, Simpson dominance index and richness index) of grass treatment were significantly higher than those of clear tillage control, and decreased with the increase of soil depth. The treatment of 0 ~ 20 cm soil layer was the highest; Compared with the control of clean tillage, the utilization intensity of six kinds of carbon source by soil microorganism treated with grasses increased significantly, and the average increase order was: carboxylic acid compound, phenol acid compound, amine compound, carbohydrate amino acid polymer. The results of principal component analysis showed that the two treatments were distributed in different regions, indicating that there was a certain metabolic difference between the two treatments, which significantly changed the function of soil microbial community. And the effect on soil microbial metabolism of carboxylic acids, carbohydrates and phenolic acids is the most obvious carbon source. The results indicated that the growth of grass was beneficial to the improvement of soil microbial community structure in vineyards.
【作者单位】：中国农业科学院郑州果树研究所;
【基金】：河南省重点科技攻关项目(152102110112) 中央级科研院所基本科研业务费专项(1610192017607) 中国农业科学院科技创新工程专项经费(CAAS-ASTIP-2017-ZFRI)
【分类号】：S154.3;S663.1

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