肉色吸水链霉菌SH121接合转移系统的建立
发布时间：2019-03-08 09:12
【摘要】：利用单因素试验探索了热激处理、预萌发时间、供体宿主菌的类型、供受比及MgCl_2浓度对链霉菌SH121接合转移效率的影响,结果显示:在50℃热激10min,37℃预萌发1h,以大肠杆菌DCDA/pUZ8002为供体宿主菌,在供受比为100∶1时,使用添加40mmol/L MgCl_2的培养基M-ISP4,接合转移效率最高可达到每受体1.03×10~(-3)个接合子。利用建立好的接合转移方法,将整合型质粒pSET152和基因敲除质粒pYZ09成功地导入链霉菌SH121,证实该方法可用于后期链霉菌SH121活性天然产物的挖掘及其相关生物合成基因簇的研究工作。
[Abstract]:The effects of heat shock treatment, pre-germination time, donor host bacteria type, donor ratio and MgCl_2 concentration on the conjugation and transfer efficiency of Streptomyces SH121 were investigated by single factor test. The results showed that after heat shock at 50 鈩，
本文编号：2436658
[Abstract]:The effects of heat shock treatment, pre-germination time, donor host bacteria type, donor ratio and MgCl_2 concentration on the conjugation and transfer efficiency of Streptomyces SH121 were investigated by single factor test. The results showed that after heat shock at 50 鈩，
本文编号：2436658
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