氨氧化菌的分离鉴定及其在罗氏沼虾育苗废水中的应用
发布时间：2019-03-16 15:41
【摘要】：氨氮转化细菌在养殖水生态系统的物质循环和水体净化的过程中起着重要作用。该研究从养殖池塘底泥中筛选具有氨氮去除能力的细菌,通过生化和分子生物学技术进行细菌鉴定,并用于罗氏沼虾育苗用水的氨氮净化。结果表明:分离获得的氨氮去除菌YX0703能在无机氮培养基和有机氮培养基中生长,既具有氨氮氧化活性,也能将NO_3~-转化为NO_2~-,再将NO_2~-转化为N_2,经鉴定,该菌为人苍白杆菌(Ochrobactrum anthropi);该菌株携带亚硝酸还原酶基因(Nitrite reductase K gene,nirK),药物最低抑菌浓度检测发现,其对头孢类抗菌素耐药,对喹诺酮类、美罗培南和四环素等抗菌药物敏感;摇床转速150 r·min~(-1)时,在25、30℃条件下,菌株YX0703对罗氏沼虾育苗废水的氨氮去除率分别为96.98%和97.44%。综上,分离获得的氨氮去除菌YX0703是人苍白杆菌,其既能利用无机氮又能利用有机氮,既具有氨氧化活性又有反硝化活性,可用于罗氏沼虾育苗废水的氨氮净化。
[Abstract]:Ammonia-nitrogen conversion bacteria play an important role in the process of material circulation and water purification in aquaculture water ecosystem. In this study, the bacteria with ammonia nitrogen removal ability were screened from the sediment of cultured ponds. The bacteria were identified by biochemical and molecular biological techniques, and were used to purify ammonia nitrogen in the water used for the breeding of Macrobrachium rosenbergii (Macrobrachium rosenbergii). The results showed that the ammonia nitrogen removal bacteria YX0703 could grow in inorganic nitrogen medium and organic nitrogen medium, which not only had ammonia nitrogen oxidation activity, but also transformed NO_3~- into NO_2~-, and then transformed NO_2~- into N\ + 2\ +\ {2\}. The results showed that the bacteria could grow in inorganic nitrogen medium and organic nitrogen medium. The bacterium is called (Ochrobactrum anthropi);. The strain carried the nitrite reductase gene (Nitrite reductase K gene,nirK). The minimal inhibitory concentration (MIC) of the drug showed that it was resistant to cephalosporins and sensitive to quinolones, meropenem and tetracycline, and was sensitive to antibiotics such as quinolones, meropenem and tetracycline. At the rotating speed of 150r 路min ~ (- 1), the ammonia nitrogen removal rate of strain YX0703 was 96.98% and 97.44% respectively at 25 鈩，
本文编号：2441659
[Abstract]:Ammonia-nitrogen conversion bacteria play an important role in the process of material circulation and water purification in aquaculture water ecosystem. In this study, the bacteria with ammonia nitrogen removal ability were screened from the sediment of cultured ponds. The bacteria were identified by biochemical and molecular biological techniques, and were used to purify ammonia nitrogen in the water used for the breeding of Macrobrachium rosenbergii (Macrobrachium rosenbergii). The results showed that the ammonia nitrogen removal bacteria YX0703 could grow in inorganic nitrogen medium and organic nitrogen medium, which not only had ammonia nitrogen oxidation activity, but also transformed NO_3~- into NO_2~-, and then transformed NO_2~- into N\ + 2\ +\ {2\}. The results showed that the bacteria could grow in inorganic nitrogen medium and organic nitrogen medium. The bacterium is called (Ochrobactrum anthropi);. The strain carried the nitrite reductase gene (Nitrite reductase K gene,nirK). The minimal inhibitory concentration (MIC) of the drug showed that it was resistant to cephalosporins and sensitive to quinolones, meropenem and tetracycline, and was sensitive to antibiotics such as quinolones, meropenem and tetracycline. At the rotating speed of 150r 路min ~ (- 1), the ammonia nitrogen removal rate of strain YX0703 was 96.98% and 97.44% respectively at 25 鈩，
本文编号：2441659
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