几种配方制备有机肥菌群分析及其育苗效果评价

发布时间：2019-05-05 18:10

【摘要】：【目的】明确几种不同配方对牛粪、羊粪、鸡粪有机肥发酵过程中菌群变化的影响及其育苗效果。【方法】以不同配方分别处理牛、羊、鸡粪,经30 d的保温、厌氧发酵制成有机肥,采用PCR-DGGE、Biolog生态板技术解析菌群变化情况及碳代谢活性;采用番茄穴盘育苗实验评价肥效。【结果】从DGGE分离菌肥微生物结果为乳酸杆菌属(Lactobacillus sp.)和魏斯氏菌属(Weissella sp.)所占比例较大,乳酸杆菌属(Lactobacillus sp.)为40.4%魏斯氏菌属(Weissella sp.)为23.8%;从平均颜色变化率(AWCD)可以看出Shannon-Wiener指数(H’)羊粪要高于鸡粪和牛粪的水平;主成分分析显示牛粪组在3个处理的菌群碳源利用水平均存在差异;羊粪组Y1和Y2菌群群落结构相对较一致,处理Y3和Y4存在差异;鸡粪组处理J2和J3群落结构相对较一致,处理J1和J4则存在差异。不同的处理方法对植株的4个指标影响各不相同,存在较大差异性。【结论】不同配方处理对牛、羊、鸡粪有机肥菌群结构造成显著影响,N3、Y3和J1处理的肥效最佳,番茄苗株高平均比对照高出25.5%、22.03%和26.16%。
[Abstract]:[objective] to determine the effect of several different formulations on the changes of microflora in the fermentation process of cattle manure, sheep manure and chicken manure and the effect of raising seedlings. [methods] cattle, sheep and chicken dung were treated with different formulas for 30 days. Anaerobic fermentation was used to produce organic fertilizer. PCR-DGGE,Biolog ecological plate technique was used to analyze the variation of flora and carbon metabolism activity. Tomato seedling experiment was used to evaluate the fertilizer efficiency. [results] the results of microbial isolation from DGGE showed that Lactobacillus spp. (Lactobacillus sp.) was found to be a member of the genus Lactobacillus. And (Weissella sp.) The large proportion of Lactobacillus belongs to (Lactobacillus sp.) 40.4% Weissella (Weissella sp.) The Shannon-Wiener index (H') of sheep manure was higher than that of chicken manure and cow manure from the average color change rate (AWCD) of 23.8%, and the results of principal component analysis showed that there was an average difference in water utilization of carbon source in three groups of bacteria treated with cow dung. The community structure of Y1 and Y2 in sheep manure group was relatively consistent, and there was difference in treatment Y3 and Y4, while the community structure of J2 and J3 in chicken manure group was relatively consistent, while there was difference in treatment J1 and J4. [conclusion] different treatments have significant effects on the microbial community structure of cattle, sheep and chicken manure organic manure, and the fertilizer effect of N _ 3, Y _ 3 and J _ 1 treatments is the best, and the treatment of N _ 3, Y _ 3 and J _ 1 has the best effect on the structure of organic fertilizer flora of cattle, sheep and chicken manure. The average plant height of tomato seedlings was 25.5%, 22.03% and 26.16% higher than that of the control.
【作者单位】：新疆农科院微生物应用研究所;
【基金】：国家自然科学基金项目“细菌纤维素对低温条件下提高甲烷产量影响机制探索”(31260016);国家自然科学基金项目“产氢菌XL1低温下提高甲烷的微生物学影响机制研究”(21666038) 新疆农业科学院青年基金项目“高效生物有机肥筛选及发酵工艺研究”(xjnkq-2015013)~~
【分类号】：S141

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