烟蚜线粒体基因组全序列及系统发育分析（英文）
发布时间：2019-05-09 01:12
【摘要】：【目的】蚜虫是杂食性农业害虫。本研究旨在通过线粒体基因组分析更好地了解蚜科昆虫的系统发育关系。【方法】结合第二代测序和PCR扩增技术获得了烟蚜Myzus persicae线粒体基因组全序列,与蚜科其他昆虫进行了对比分析;以贝叶斯法和最大似然法基于13个蛋白编码基因对蚜科进行了系统发育分析。【结果】烟蚜线粒体基因组(Gen Bank登录号:KU_236024)序列全长17 832 bp,A+T含量84.1%,AT偏斜为0.094,GC偏斜为-0.296。包含13个蛋白编码基因(cox1-3,nad1-6,nad4L,atp6,atp8和cytb),22个tRNA,2个rRNA基因(rrn L和rrn S)和2个长的非编码区,其基因排列顺序与已知的蚜科昆虫相似,除了nad4以单独的T结尾,所有的蛋白编码基因均以ATN作为起始密码子,TAA作为终止密码子。在烟蚜线粒体基因组中,tRNAGlu和tRNAPhe中间有一段307 bp的非编码区,该编码区包含2个重复单元,烟蚜的控制区长2 531 bp,是所有测序蚜虫线粒体基因组中最长的。rrn L的二级结构包含6个结构域,44个茎环结构;rrn S的二级结构有3个结构域,24个茎环结构。基于烟粉虱和其他20种昆虫的13个蛋白编码基因重建的BI和ML系统发育树,与传统形态学分类结果一致。【结论】蚜亚科和长管蚜亚科的单系性得到了很好的支持;在长管蚜亚科的分支中,M.persicae与D.noxia聚成一支,并且C.salicicola位于进化枝的底部。本研究结果为蚜科系统发生关系重建积累了有价值的数据资料。
[Abstract]:[objective] aphids are omnivorous agricultural pests. The aim of this study was to better understand the phylogenetic relationship of aphids by mitochondrial genome analysis. [methods] complete mitochondrial genome sequences of aphid Myzus persicae were obtained by using the second generation sequencing and PCR amplification techniques. The results were compared with other insects of aphids. Phylogenetic analysis of aphids was carried out by using Bayesian method and maximum likelihood method based on 13 protein coding genes. [results] the total length of mitochondrial (Gen Bank accession number: KU_236024) sequence of APHIS fumigatus was 17832 bp,A T content 84.1%. The deviation of AT and GC were 0.094 and-0.296 respectively. It contains 13 protein-coding genes (cox1-3,nad1-6,nad4L,atp6,atp8 and cytb), 22 tRNA,2, (rrn L and rrn S), and 2 long non-coding regions), the sequence of which is similar to that of known aphids. Except that nad4 ends with a single T, all the protein coding genes use ATN as the starting codon and TAA as the terminating codon. In the mitochondrial genome of APHIS fumigatus, there is a non-coding region of 307bp between tRNAGlu and tRNAPhe. The coding region contains two repeat units, and the control region of APHIS fumigatus is 2531 bp,. The secondary structure of rrn L contains 6 domains and 44 stem rings. The secondary structure of rrn S has 3 domains and 24 stem rings. The phylogenetic tree of BI and ML based on 13 protein coding genes of Bemisia Tabaci and other 20 species of insects is consistent with the results of traditional morphological classification. [conclusion] the monophyletic characters of aphid subfamily and long tube aphid subfamily are well supported. In the branches of the aphids, M.persicae and D.noxia form one branch, and C.salicicola is located at the bottom of the evolutionary branch. The results of this study have accumulated valuable data for the phylogenetic relationship reconstruction of aphids.
【作者单位】： 贵州大学烟草学院;贵州大学昆虫研究所;贵州省烟草公司毕节市公司;
【基金】：贵州省烟草公司毕节市公司科技项目“毕节市烟蚜捕食性天敌生防体系的构建与应用”([2016]82);贵州省烟草公司科技项目“烟蚜优效捕食性天敌应用技术研究”([2015]11) 贵州省科技厅项目“贵州省烟草品质遗传改良与生物转化科技创新人才团队建设”[黔科合人才团队(2015)4011]
【分类号】：S433
[Abstract]:[objective] aphids are omnivorous agricultural pests. The aim of this study was to better understand the phylogenetic relationship of aphids by mitochondrial genome analysis. [methods] complete mitochondrial genome sequences of aphid Myzus persicae were obtained by using the second generation sequencing and PCR amplification techniques. The results were compared with other insects of aphids. Phylogenetic analysis of aphids was carried out by using Bayesian method and maximum likelihood method based on 13 protein coding genes. [results] the total length of mitochondrial (Gen Bank accession number: KU_236024) sequence of APHIS fumigatus was 17832 bp,A T content 84.1%. The deviation of AT and GC were 0.094 and-0.296 respectively. It contains 13 protein-coding genes (cox1-3,nad1-6,nad4L,atp6,atp8 and cytb), 22 tRNA,2, (rrn L and rrn S), and 2 long non-coding regions), the sequence of which is similar to that of known aphids. Except that nad4 ends with a single T, all the protein coding genes use ATN as the starting codon and TAA as the terminating codon. In the mitochondrial genome of APHIS fumigatus, there is a non-coding region of 307bp between tRNAGlu and tRNAPhe. The coding region contains two repeat units, and the control region of APHIS fumigatus is 2531 bp,. The secondary structure of rrn L contains 6 domains and 44 stem rings. The secondary structure of rrn S has 3 domains and 24 stem rings. The phylogenetic tree of BI and ML based on 13 protein coding genes of Bemisia Tabaci and other 20 species of insects is consistent with the results of traditional morphological classification. [conclusion] the monophyletic characters of aphid subfamily and long tube aphid subfamily are well supported. In the branches of the aphids, M.persicae and D.noxia form one branch, and C.salicicola is located at the bottom of the evolutionary branch. The results of this study have accumulated valuable data for the phylogenetic relationship reconstruction of aphids.
【作者单位】： 贵州大学烟草学院;贵州大学昆虫研究所;贵州省烟草公司毕节市公司;
【基金】：贵州省烟草公司毕节市公司科技项目“毕节市烟蚜捕食性天敌生防体系的构建与应用”([2016]82);贵州省烟草公司科技项目“烟蚜优效捕食性天敌应用技术研究”([2015]11) 贵州省科技厅项目“贵州省烟草品质遗传改良与生物转化科技创新人才团队建设”[黔科合人才团队(2015)4011]
【分类号】：S433
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