土壤可溶性氮及氮代谢酶活性动态变化及对温度的响应
发布时间：2019-08-11 20:49
【摘要】：采用室内恒湿培养试验探讨土壤可溶性氮及氮代谢酶活性的动态变化规律及温度对土壤可溶性氮及氮代谢酶活性的影响。结果表明,土壤NO_3~--N含量呈先增加后降低的趋势,在培养48~58d达到最大值;NH+4-N含量呈降低趋势,培养73d后趋于稳定;SON和TSN含量呈"升高-降低-升高-降低"的波浪形变化趋势,在48d时累积量达到最大值。土壤NO_3~--N含量随培养温度升高显著增加,并与之达到极显著正相关;土壤NH_4~+-N则显著降低,与培养温度之间显著负相关;培养中期及培养后期部分取样时间内,土壤SON与培养温度显著相关,其他取样时间均无显著相关性。随着培养时间的延长,土壤脲酶活性呈"升-降-升-降"的抛物线动态变化趋势;土壤蛋白酶活性表现为培养前期、中期高于培养后期;土壤天冬酰胺酶活性呈折线上升后下降的变化规律;土壤天谷氨酰胺酶活性呈折线上升的变化趋势。从培养温度看,在本研究设定的温度范围内,土壤温度的变化对脲酶活性无显著影响,但在部分取样时间内对蛋白酶、天冬酰胺酶和谷氨酰胺酶活性有显著影响。
[Abstract]:The dynamic changes of soil soluble nitrogen and nitrogen metabolizing enzyme activity and the effect of temperature on soil soluble nitrogen and nitrogen metabolizing enzyme activity were studied by laboratory constant humidity culture experiment. The results showed that the content of NO_3~--N in soil increased at first and then decreased, and reached the maximum at 48 and 58 days of culture, the content of NH 4 showed a decreasing trend and tended to be stable after 73 days of culture, and the content of SON and TSN showed a wave shape change trend of "increase, decrease, increase and decrease", and the accumulation reached the maximum at 48 days. Soil NO_3~--N content increased significantly with the increase of culture temperature, and reached a very significant positive correlation with it, while soil NH_4~-N decreased significantly and negatively correlated with culture temperature. Soil SON was significantly correlated with culture temperature in the middle and later stages of culture, but there was no significant correlation between other sampling time. With the prolongation of culture time, the activity of soil urease showed a parabola dynamic trend of "rise-fall-rise-fall", the activity of soil protease was higher in the early stage and middle stage of culture than in the later stage of culture, the activity of asparaginase in soil decreased after the increase of broken line, and the activity of asparaginase in soil showed the trend of increasing in line. From the point of view of culture temperature, the change of soil temperature had no significant effect on urease activity, but had significant effect on protease, asparaginase and glutaminase activities in some sampling time.
【作者单位】： 福建省农业科学院农业生态研究所;福建省丘陵地区循环农业工程技术研究中心;福建农林大学资源环境学院;
【基金】：福建省科技计划项目——省属公益类科研院所基本科研专项(2015R1017-2、2016R1016-2、2015R1017-1) 福建省农业科学院青年人才创新基金项目(YC2016-12)
【分类号】：S151.9
，
本文编号：2525510
[Abstract]:The dynamic changes of soil soluble nitrogen and nitrogen metabolizing enzyme activity and the effect of temperature on soil soluble nitrogen and nitrogen metabolizing enzyme activity were studied by laboratory constant humidity culture experiment. The results showed that the content of NO_3~--N in soil increased at first and then decreased, and reached the maximum at 48 and 58 days of culture, the content of NH 4 showed a decreasing trend and tended to be stable after 73 days of culture, and the content of SON and TSN showed a wave shape change trend of "increase, decrease, increase and decrease", and the accumulation reached the maximum at 48 days. Soil NO_3~--N content increased significantly with the increase of culture temperature, and reached a very significant positive correlation with it, while soil NH_4~-N decreased significantly and negatively correlated with culture temperature. Soil SON was significantly correlated with culture temperature in the middle and later stages of culture, but there was no significant correlation between other sampling time. With the prolongation of culture time, the activity of soil urease showed a parabola dynamic trend of "rise-fall-rise-fall", the activity of soil protease was higher in the early stage and middle stage of culture than in the later stage of culture, the activity of asparaginase in soil decreased after the increase of broken line, and the activity of asparaginase in soil showed the trend of increasing in line. From the point of view of culture temperature, the change of soil temperature had no significant effect on urease activity, but had significant effect on protease, asparaginase and glutaminase activities in some sampling time.
【作者单位】： 福建省农业科学院农业生态研究所;福建省丘陵地区循环农业工程技术研究中心;福建农林大学资源环境学院;
【基金】：福建省科技计划项目——省属公益类科研院所基本科研专项(2015R1017-2、2016R1016-2、2015R1017-1) 福建省农业科学院青年人才创新基金项目(YC2016-12)
【分类号】：S151.9
，
本文编号：2525510
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