黄芩苷在黄芩植株体内积累规律的研究

发布时间：2018-01-01 03:30

  本文关键词：黄芩苷在黄芩植株体内积累规律的研究　出处：《山东中医药大学》2004年硕士论文　论文类型：学位论文

  更多相关文章： 黄芩 黄芩苷 外界因素 POD酶 蛋白质

【摘要】：为了解黄芩植株体内黄芩苷的合成积累规律及其影响因素，达到控制或提高栽培药材质量的目的，我们采用种子发芽与盆栽方式，利用高效毛细管电泳、高效液相色谱等手段，研究了黄芩苷在黄芩植株各器官中的分布及生长时间、温度、光照、营养元素等因素对黄芩苷含量的影响。结果表明：黄芩种子不含黄芩苷，植株其它器官中均含有，但含量多少依次为：根花叶茎，主根侧根支根，韧皮部木质部；种子萌发至子叶展开时，黄芩苷才开始产生，随着幼苗不断长大含量逐步提高，一年生植株的生长时间越长，根中黄芩苷的含量越高：光照具有促进黄芩苷合成的作用，光照条件下生长幼苗中的黄芩苷含量显著高于黑暗条件下生长者，光照越强，植株根部的黄芩苷含量越高；温度对黄芩苷的合成也有影响，以25℃最适。补充营养元素能促进黄芩苷合成，在种子发芽时以钾肥和氮磷复合肥的促进作用最大，对砂培植株来讲，铜有抑制作用，，而磷、钾等元素有促进作用；在植株营养状况较差的情况下，补充蔗糖与葡萄糖能够提高黄芩苷含量，正常植株补充蔗糖与葡萄糖时，黄芩苷的含量有所降低；黄芩苷含量与可溶性蛋白质含量之间具有一定的相关性，在种子萌发幼苗生长期间为明显的负相关性，在施肥补充营养元素后为明显的正相关性：各器官均有过氧化物酶存在，但POD酶谱中的带1，只存在于根中，在茎、叶及花中存在较少或不存在，既使在根中也会因生长时间不同而存在谱带宽度的差异；不同产地种子的蛋白电泳图谱有明显差异，证明其遗传性发生了变化。
[Abstract]:In order to understand the synthesis and accumulation of baicalin in Scutellaria baicalensis plant and its influencing factors, and to control or improve the quality of cultivated medicinal materials, we adopted seed germination and pot culture, and used high performance capillary electrophoresis (HPCE). The distribution, growth time, temperature and illumination of baicalin in various organs of Scutellaria baicalensis were studied by high performance liquid chromatography (HPLC). The results showed that baicalin was not contained in the seeds of Scutellaria baicalensis, but was found in other organs of the plant, but the contents were in order as follows: root, flower, leaf stem, main root, lateral root branch and root. Phloem xylem; Baicalin began to produce only when the seeds germinated to cotyledon development, and the growing time of annual plants increased with the increasing of seedling growth. The higher the content of baicalin in the root: light can promote the synthesis of baicalin, the higher the content of baicalin in the growth seedlings was, the stronger the light was. The higher the content of baicalin in the root; Temperature also had an effect on the synthesis of baicalin, and the optimum temperature was 25 鈩

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