真菌诱导子对人参毛状根皂苷生物合成和生长的影响

发布时间：2018-03-04 06:03

本文选题：真菌诱导子　切入点：人参毛状根　出处：《中国中药杂志》2004年04期 　论文类型：期刊论文

【摘要】：目的 :考察真菌诱导子对人参毛状根的生长和人参皂苷生物合成影响。方法 :提取黄瓜炭疽病菌Colletorichumlagnarinm、青菜炭疽病菌Phomafiltrate、棉花枯萎病菌Fusariumoxysponum、黑曲霉Asperillusniger中的真菌诱导子与人参毛状根共培养 ,应用HPLC及比色法对培养物中总苷及几种单体皂苷进行分析测定。结果 :真菌诱导子不但能影响人参毛状根总苷的合成量 ,也能使某些单体皂苷消失或增加 ,如培养液中黑曲霉多糖诱导子增加到 2 0mg·L-1浓度时 ,使总苷含量增加到 3.6 4 9% ,而单体皂苷中Rg1和Re未检出 ,Rg2 和Rb1的含量则有明显增加 ,并可促进人参毛状根的生长。结论 :真菌诱导子对人参毛状根某些皂苷的合成具有特异性 ,同时也影响人参毛状根的生物量。培养过程中通过外源性诱导子的添加 ,有利于人参毛状根次生代谢产物的定向积累
[Abstract]:Objective: to investigate the effects of fungal elicitors on the growth of hairy roots of ginseng and the biosynthesis of ginsenoside. Methods: Colletorichumlagnarinm, Phomafiltrate, Fusarium oxysponumand Asperillusniger of Aspergillus Niger were extracted from Cucumber anthracnose, Phomafiltrate, Fusarium oxysponum, and Aspergillus Niger, respectively. Co culture with ginseng hairy root, The total glycosides and several monomers saponins in the culture were determined by HPLC and colorimetry. Results: the fungal elicitor could not only influence the synthesis of the total glycosides in the hairy roots of ginseng, but also make some monomer saponins disappear or increase. When the concentration of Aspergillus Niger polysaccharides was increased to 20 mg 路L -1, the content of total glycosides increased to 3.64 9%, while the contents of Rg1 and re in the monosaponins increased significantly. Conclusion: the fungal elicitor is specific to the synthesis of some saponins in the hairy roots of Panax ginseng, and it also affects the biomass of the hairy roots of ginseng. Directional accumulation of secondary metabolites in hairy roots of Panax ginseng
【作者单位】：中国药科大学中药学院中国药科大学中药学院中国药科大学中药学院中国药科大学中药学院中国药科大学中药学院
【分类号】：S567.51

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