嵌套循环生物膜法降解含聚污水研究

发布时间：2018-04-09 07:46

本文选题：聚丙烯酰胺　切入点：降解　出处：《东北石油大学》2015年硕士论文

【摘要】：聚丙烯酰胺已广泛应用于三次采油,导致油田产出水聚合物含量超标,对油田环境造成污染,因而对聚丙烯酰胺污水进行处理具有重要意义。生物法降解聚丙烯酰胺具有成本低、二次污染小等优点,得到了研究人员广泛关注。本文采用一种嵌套循环生物膜法处理含聚污水,构建了内循环嵌套外循环的双循环模式,使得废水在空间上交替流经缺氧环境和好氧环境,以实现聚丙烯酰胺废水有机物和氮素的有效去除。本文根据载体应具备的性质,选择合适载体,确定载体投加量,启动嵌套循环生物膜反应器。研究好氧区聚丙烯酰胺浓度和水力停留时间(HRT)对聚丙烯酰胺废水中有机物去除效率的影响。通过向进水中加入辅酶前体维生素B族生长因子和葡萄糖、淀粉、蛋白胨等碳源和氮源强化嵌套循环生物膜法对聚丙烯酰胺的去除效能。探讨嵌套循环模式、进水方式和内循环表观气速与脱氮效能内在关系。对载体进行生物相观察,对聚丙烯酰胺降解菌进行筛选,并初步研究金属离子对聚丙烯酰胺降解菌降解特性的影响。此外,研究嵌套循环生物膜法水动力学,并测定嵌套循环生物膜法生物动力学系数。研究结果表明,经60天驯化后,平均COD去除率达到70.37%。驯化期间在聚丙烯酰胺浓度升高初期,COD去除率逐渐下降,HRT24h时,COD去除率随HRT增大而升高,HRT24h后COD去除率趋于平稳,确定最佳HRT为24h。提高载体浓度可增大生物量,COD去除效能提高,B族维生素对聚丙烯酰胺去除率影响较小,葡萄糖和淀粉可使去除率分别提高7%和6%,蛋白胨对去除率影响较大,去除率可从60%提高到75%。氮素去除实验表明,采用从缺氧区进水方式,在内循环表观气速为0.003mg/L条件下,嵌套循环脱氮效率较高,氨氮去除率为85%,总氮去除率为33.3%。镜检结果显示好氧区载体生物膜生物相结构稳定,生物膜由大量丝状菌和少量细菌组成。分离筛选出两株为杆状革兰氏阴性菌的聚丙烯酰胺降解菌P1和P2,并且金属离子Mg2+和Zn2+对菌种生长和PAM降解有促进作用,但Mn2+对P1和P2有一定程度抑制作用。动力学研究结果表明,好氧区基质可在1min内完成混合,为连续流完全混合型,外循环曝气量与气含率之间成线性关系,拟合方程为y=10.725x-0.504,外循环气含率与循环流速的平方成类线性关系。选定气提管顶部处为最佳进水口位置,建立水动力学模型,解得最小临界气速为Ugr=0.76m/s。实验测定嵌套循环好氧区颗粒生物膜产率系数为Y=0.40645(mg/m3)/(mg/L PAM)。
[Abstract]:Polyacrylamide has been widely used in the extraction of three times, to exceed the standard of oilfield produced water polymer content, oil pollution to the environment, so it is of great significance for processing wastewater. The degradation of polyacrylamide polyacrylamide biological method has the advantages of low cost, small two pollution and other advantages, has been widely concerned by researchers. In this paper, a nested loop biofilm treatment of wastewater containing polymer, constructed in the nested loop outer loop double loop mode, so that waste water flows through the alternating aerobic anoxic environment in the space environment, to achieve effective removal of polyacrylamide wastewater organic matter and nitrogen. According to the nature of support should have the appropriate carrier, determine the carrier dosage, start the nested loop biofilm reactor. The aerobic zone of polyacrylamide concentration and hydraulic retention time (HRT) on the removal efficiency of organic compounds in wastewater of polyacrylamide The effect by adding coenzyme precursor B vitamins and growth factors to influent glucose, starch, peptone and efficiency of carbon source and nitrogen source to strengthen the nested loop biofilm removal of polyacrylamide. To investigate the nested loop mode, feeding mode and the inner circulation of superficial gas velocity and the nitrogen removal efficiency of biological internal relations. Observation of the carrier phase, the polyacrylamide degrading bacteria were screened, and a preliminary study on the effect of metal ions on the degradation of polyacrylamide degrading bacteria. In addition, research on nested loop biofilm water dynamics, and the determination of the nested loop biofilm kinetic coefficient. The results show that after 60 days of acclimation, the average removal rate of COD reached 70.37%. during the period of acclimation increased in the early COD removal rate of polyacrylamide concentration, decreased HRT24h, the removal rate of COD with HRT increased, HRT24h removal rate of COD tends to be stable, determined The best HRT can increase the biomass of 24h. to improve the carrier concentration, COD removal efficiency increased, vitamin B has little effect on the removal rate of polyacrylamide, glucose and starch can improve the removal rate of 7% and 6% respectively, peptone had great influence on the removal rate, the removal rate can be increased from 60% to 75%. The nitrogen removal experiments showed that using from anoxic influent modes, including circulating superficial gas velocity is 0.003mg/L, nested loops for higher efficiency of nitrogen removal, ammonia nitrogen removal rate was 85%, total nitrogen removal rate of 33.3%. microscopy results showed that the aerobic zone carrier biofilm structure, biological membranes are composed of a large number of filamentous bacteria and a small amount of bacteria isolated. Two strains of rod-shaped gram negative bacteria polyacrylamide degrading bacteria P1 and P2, and the metal ions Mg2+ and Zn2+ have positive effects on the growth and PAM degradation, but Mn2+ has a certain degree of inhibition of P1 and P2 dynamics. The results of the study show that aerobic zone matrix mixing can be completed within 1min for continuous flow completely mixed, circulating aeration and gas holdup between the linear equation is y=10.725x-0.504, circulating gas holdup and circulation velocity as the square of linear relation. The selected stripping tube at the top for the best position of the water inlet the establishment of water, kinetic model, solution of minimum critical gas velocity determination of nested loop aerobic granular biofilm yield coefficient of Ugr=0.76m/s. experiment Y=0.40645 (mg/m3) / (mg/L PAM).

【学位授予单位】：东北石油大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：X741

【相似文献】