石油炼化废水组成对厌氧氨氧化-反硝化细菌混培物的影响

发布时间：2018-05-15 02:28

本文选题：石油炼化废水 + 厌氧氨氧化　；参考：《环境工程学报》2017年04期

【摘要】：为了探究石油炼化废水中COD和毒性物质对厌氧氨氧化-反硝化细菌混培物的影响,利用已具有高效脱氮性能的细菌混培物建立生物脱氮反应器进行连续驯化实验,实现进水COD和毒性物质比例的增加,并结合MPN-PCR技术对驯化前后两类菌群数量进行检测。结果发现,驯化前后厌氧氨氧化细菌数目由7.549×10~(14)个·g~(-1)减为8.212×10~8个·g~(-1),脱氮生化活性仍保持在40.2%左右;反硝化细菌数目由3.523×10~6个·g~(-1)增为4.693×10~(16)个·g~(-1),脱氮生化活性增加了5.76倍左右。结果表明,厌氧氨氧化细菌和反硝化细菌混培脱氮体系的脱氮生化活性未与细菌数目呈正相关性变化,COD和毒性物质对体系产生了不同程度的影响;厌氧氨氧化细菌比反硝化细菌对石油炼化废水毒性的作用更敏感;混培脱氮体系在一定程度上可以有效地抵抗石油炼化废水高浓度COD、高毒性物质对厌氧氨氧化生理生化脱氮过程的负面影响。
[Abstract]:In order to investigate the effect of COD and toxic substances in petroleum refinery wastewater on anaerobic ammonia oxidation-denitrifying bacteria mixed culture, a biological denitrification reactor was established by using bacteria mixed culture with high denitrification performance. The ratio of influent COD and toxic substances was increased, and the number of two groups of bacteria before and after acclimation was detected by MPN-PCR technique. The results showed that before and after acclimation, the number of anaerobic ammonia-oxidizing bacteria decreased from 7.549 脳 10 ~ (1) to 8.212 脳 10 ~ (8) g ~ (-1), the denitrification bioactivity remained about 40.2%, the number of denitrifying bacteria increased from 3.523 脳 10 ~ 6 g ~ (-1) to 4.693 脳 10 ~ (16) g ~ (-1), and the biochemical activity of denitrification increased by 5.76 times. The results showed that the denitrification bioactivity of anaerobic ammonia-oxidizing bacteria and denitrifying bacteria was not positively correlated with the number of bacteria. COD and toxic substances had different effects on the system. Anaerobic ammonia-oxidizing bacteria are more sensitive to the toxicity of petroleum refinery wastewater than denitrifying bacteria. The mixed culture nitrogen removal system can effectively resist the negative effects of high concentration of COD and high toxic substances on the physiological and biochemical denitrification process of anaerobic ammonia oxidation.
【作者单位】：北京工业大学水质科学与水环境恢复工程北京市重点实验室;中国石油集团公司安全环保技术研究院;
【基金】：基金项目:安全环保关键技术研究与推广(2013D-4606-02)
【分类号】：X742

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