四路荧光采集系统

发布时间：2018-01-06 16:32

本文关键词：四路荧光采集系统　出处：《山东师范大学》2017年硕士论文　论文类型：学位论文

【摘要】：本课题针对基于微流控芯片中的多组分荧光同时检测要求,目前针对激光诱导荧光微流控芯片的仪器多针对单组份测量,无法实现多组分的快速同时测量。本课题就是基于这一现实状况下进行研究设计的。由于用不同波长的激光照射染色后的细胞发出的荧光比较微弱,因此如何对细胞荧光这一微弱信号进行检测是这套仪器能否准确工作的关键。这是由生物化学研究作为理论依据的,利用电子工程技术中的电路设计和软件编程支持其完成实际的应用功能,所以这是一个多学科相互融合的工程项目。本设计是利用光电倍增管(PMT)对光信号进行采集,光电倍增管将采集到的荧光数据经过I/V转换送到信号调理板,经过对荧光信号的滤波和放大后,送至数据采集卡,数据采集卡将采集到的信号传至上位机,上位机将采集到的数据以Excel表格的形式存储到电脑硬盘,将数据进行处理之后就会得到细胞的数量和细胞内成分等信息。本文章主要设计了一套高采集速率,高精度,多分组同时采集的一体化采集设备,这套系统实现了对细胞荧光信号采集以及自动化处理。本文章主要分为了六个部分,具体如下:第一,文章的绪论部分主要包括四路荧光采集系统的课题来源,研究课题的背景及意义,国内外的发展现状。第二,系统的总体设计,讲述了对系统设计方案的可行性研究,介绍了系统的基本功能及元件选型的分析。第三,这部分介绍了系统的详细设计方案,包括系统电源的设计,PMT控制模块的设计,信号调理模块的设计,AD数据采集模块的设计,主程序软件的设计,PCB电路板的设计与制作。第四,上位机的设计,包括了对LabView系统开发平台的简单介绍,还包括了主控制界面软件的设计。第五,主要介绍了对仪器的使用方法和对细胞荧光数据的采集结果。最后,本章对现在完成的工作做了总结,然后对后续系统的进一步改进做了展望。
[Abstract]:This paper aims at the requirement of multicomponent fluorescence detection based on microfluidic chip. At present, the instruments for laser-induced fluorescence microfluidic chip are mostly aimed at single component measurement. It is impossible to realize the rapid simultaneous measurement of multicomponent. This subject is based on this reality. Because of the different wavelength of laser irradiation the fluorescence emitted by the cells is relatively weak. Therefore, how to detect the weak signal of cell fluorescence is the key to the accuracy of the instrument, which is based on biochemical research. Using the circuit design and software programming in the electronic engineering technology to support it to complete the actual application function. So this is a multidisciplinary fusion of engineering projects. This design is to use photomultiplier tube (PMT) to collect optical signals. The photomultiplier tube converts the collected fluorescence data to the signal conditioning board through I / V conversion. After filtering and amplifying the fluorescence signal, it is sent to the data acquisition card, which transmits the collected signal to the upper computer. The data collected by the host computer is stored in the form of Excel form to the hard disk of the computer. After processing the data, we will get the information of cell number and intracellular components. This paper mainly designed a set of integrated acquisition equipment with high acquisition rate, high precision and multi-group collection at the same time. This system has realized the acquisition of cell fluorescence signal and automatic processing. This article is mainly divided into six parts, the details are as follows: first, the introduction of the article mainly includes four sources of fluorescence acquisition system. The background and significance of the research, the development of the status quo at home and abroad. Second, the overall design of the system, described the feasibility of the system design scheme, introduced the basic functions of the system and component selection analysis. Third. This part introduces the detailed design of the system, including the system power supply design PMT control module design, signal conditioning module design and AD data acquisition module design, the main program software design. The design and manufacture of PCB circuit board. 4th, the design of host computer, including the simple introduction of LabView system development platform, and the design of main control interface software. 5th. This paper mainly introduces the use of the instrument and the collection results of the cell fluorescence data. Finally, this chapter summarizes the work done now, and then the further improvement of the follow-up system is prospected.
【学位授予单位】：山东师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TP274.2

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