利用交流阻抗传感技术实时监测纳米沟槽上皮肤细胞的定向行为

发布时间：2018-01-21 16:41

本文关键词： 纳米沟槽定向行为交流阻抗皮肤细胞复合型细胞传感器　出处：《化学学报》2017年11期 　论文类型：期刊论文

【摘要】：细胞定向行为在皮肤、骨骼等修复和再生中起着非常重要的作用.本文首次采用交流阻抗传感技术实时监测人真皮成纤维细胞(HFF)和人永生化表皮细胞(Ha Ca T)在纳米沟槽(宽度:200 nm,周期:400 nm,深度:70 nm)上的定向行为.结果表明,HFF细胞在纳米沟槽上先进行定向排列,再发生胞体的延长;Ha Ca T细胞无定向行为的产生,其粘附和铺展得到了延缓.与平面电极相比,HFF细胞在纳米沟槽上产生的交流阻抗信号(NI值)更大,前期定向排列比后期胞体延长引起的NI值变化更显著,且NI值与定向排列的细胞百分比之间存在着良好的线性关系;Ha Ca T细胞在纳米沟槽上的NI值更小,且粘附比铺展过程对NI值变化的影响更大.本文的研究将为复合型细胞传感器的发展提供思路和支持.
[Abstract]:Cell orientation behavior in the skin. Bone repair and regeneration play a very important role. In this paper, the first real time monitoring of human dermal fibroblasts (HFFs) and human immortalized epidermal cells (Ha-Ca T) using AC impedance sensing technique was carried out. In nanogrooves (width: 200 nm). The results showed that the HFF cells were orientated first and then prolonged on the nanogutaneous grooves at the depth of 70 nm, and the cell cycle was 400 nm at the depth of 70 nms. the results showed that the HFF cells were orientated first and then the cell bodies were lengthened. The adhesion and spreading of Ha Ca T cells were delayed, and the AC impedance signal (NI value) of HFF cells on nano-grooves was larger than that of planar electrodes. The change of NI value in the early stage was more significant than that in the late stage, and there was a good linear relationship between the NI value and the percentage of the cells in the orientation arrangement. The NI value of Ha Ca T cells on nano-grooves is smaller, and the effect of adhesion ratio spreading on NI values is greater. The research in this paper will provide ideas and support for the development of complex cell sensors.
【作者单位】：华东师范大学化学与分子工程学院;
【基金】：国家自然科学基金(No.21405049)资助~~
【分类号】：R318;TP212.3
【正文快照】： Received July 26,2017;published September 8,2017.Supporting information for this article is available free of charge via the Internet at http://sioc-journal.cnProject supported by the National Natural Science Foundation of China(No.21405049).项目受国家自

【相似文献】