碳点荧光传感器的制备及其性能研究

发布时间：2018-03-08 06:32

本文选题：碳纳米点　切入点：荧光传感器　出处：《天津工业大学》2017年硕士论文　论文类型：学位论文

【摘要】：最近,碳点(CDs)作为优越的荧光团出现,有很多吸引人的优点,包括优异的光稳定性、尺寸小、生物相容性好、高度可调光致发光属性、电化学发光、化学惰性、易于与分子功能化等。碳点作为化学传感器,用于重金属离子和有机物的检测。本文的主要工作如下:1.生物质是制备杂原子掺杂的碳纳米材料的良好的前体。我们用鸽子羽毛、蛋和粪作为前体,通过热解碳化的方法制备氮硫掺杂的荧光碳点(PCDs),制备的PCDs都具有较高的荧光量子产率分别是24.87%(PCDs-f)、17.48%(PCDs-w)、16.34%(PCDs-y)和 33.50%(PCDs-m)。我们发现 PCDs-m对重金属离子没有单一的选择性,其他的PCDs对Hg~(2+)/Fe_(3+)有较高的灵敏性和选择性,同时PCDs-f的最低检测限为10.3(Hg~(2+))和60.9nM(Fe_(3+))。能够用于人脐静脉内皮细胞的成像,表明碳点的低毒性和较好的生物相容性。2.氮和硼掺杂的碳点(BCNDs1-3)的制备是从三种不同的硼酸通过水热法合成的。BCNDs拥有良好的水溶性和较高的荧光量子产率分别为29.01%、51.42%和68.28%。随后,BCNDs作为H2+和三硝基苯酚(TNP)良好的的探针。有效的选择性检测Hf+可以归因于通过有效的电子转移过程促进BCNDs无辐射电子/空穴复合湮灭和TNP的检测可以归因于荧光共振能量转移过程。结果表明BCNDs2对Hg~(2+)的和TNP的检测是最灵敏的荧光探针。3.碳点与4-羧基苯硼酸(CPBA)共价结合,合成4-羧基苯硼酸功能化的碳点(CPBA-CDs)。CPBA-CDs能与邻苯二酚的邻二醇结构共价结合,主要是通过表面淬火诱导机制,从而使CPBA-CDs的荧光猝灭。检测邻苯二酚的线性范围为0.1-56 μM检测限为115 nM。根据CPBA-CDs荧光发射光谱可以实现在细胞里多色成像。4.溴乙酰溴功能化的碳点(CDs-Br)用于直接检测谷胱甘肽(GSH)。溴乙酰溴功能化的部分(卤素部分)能够使CDs-Br的荧光发生猝灭,然而这个卤素部分可以被巯基取代,从而是CDs-Br的荧光恢复。在优化的条件下,探针CDs-Br 对谷胱甘肽有良好的选择性,超过了半胱氨酸和高半胱氨酸。同时可以用于细胞里面检测谷胱甘肽和细胞成像。
[Abstract]:Recently, carbon dots have emerged as superior fluorescent groups with many attractive advantages, including excellent photostability, small size, good biocompatibility, highly tunable photoluminescence properties, electrochemiluminescence, chemical inertia, Carbon spots are used as chemical sensors for the detection of heavy metal ions and organic compounds. The main work of this paper is as follows: 1.Biomass is a good precursor for the preparation of hetero-atom-doped carbon nanomaterials. We use pigeon feathers, Egg and feces were used as precursors to prepare nitrogen-sulfur doped fluorocarbon dots by pyrolytic carbonization. The PCDs obtained by pyrolysis and carbonation had high fluorescence quantum yields of 24.87% and 17.48% respectively. We found that PCDs-m had no single selectivity for heavy metal ions. The other PCDs have high sensitivity and selectivity for Hg~(2 / FeS-1), and the minimum detection limit for PCDs-f is 10.3hgf2) and 60.9nM / FeS-1, which can be used in the imaging of human umbilical vein endothelial cells (HUVECs). The results show that the low toxicity and good biocompatibility of carbon dots. 2. The preparation of nitrogen and boron doped carbon dots BCNDs1-3). BCNDs synthesized by hydrothermal method from three different boric acids have good water solubility and high fluorescence quantum yield of 29.01%. Then BCNDs were good probes for H2 and trinitrophenol. The effective selective detection of HF can be attributed to the combination of radiation free electron / hole annihilation and TNP detection through effective electron transfer process. Because of the fluorescence resonance energy transfer process, the results show that the detection of Hg~(2 by BCNDs2 and TNP is the most sensitive fluorescence probe .3.The carbon point is covalently bound to 4-carboxyphenylborate. The synthesis of 4-carboxyphenylboric acid functionalized carbon dot CPBA-CDsN. CPBA-CDs can bind covalently with the structure of catechol, mainly through the mechanism of surface quenching induction. The linear range of detection of catechol is from 0.1 to 56 渭 M. according to the fluorescence emission spectrum of CPBA-CDs, it can be realized in the cell of polychromatic imaging .4. the bromoacetyl bromide functionalized carbon dot CDs-Br) can be used to detect the valley directly. The functionalized part of bromoacetyl bromide (halogen part) can quench the fluorescence of CDs-Br. However, this halogen can be replaced by sulfhydryl, which is the fluorescence recovery of CDs-Br. Under optimized conditions, the probe CDs-Br has good selectivity for glutathione. More than cysteine and homocysteine. Can also be used in cell detection of glutathione and cell imaging.
【学位授予单位】：天津工业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TP212

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