基于拟糖蛋白的电化学阻抗生物传感器用于糖与蛋白质相互作用的研究
发布时间：2019-02-14 19:13
【摘要】：以D-甘露糖和伴刀豆球蛋白(ConA)的相互作用为研究对象,利用美拉德反应将D-甘露糖共价结合到负载蛋白牛血清蛋白(BSA)的表面形成拟糖蛋白,然后将拟糖蛋白固定到玻碳电极表面,以拟糖蛋白表面的D-甘露糖为分子识别物质,构建了检测伴刀豆球蛋白(ConA)的电化学阻抗传感器。拟糖蛋白制备过程简单,D-甘露糖负载量大,在空间中提供多个结合位点,因此能与ConA形成多价复合物,提高了传感器的灵敏度。该传感器的响应值与ConA浓度的对数在5.0×10-11~5.0×10-9mol/L之间呈良好的线性关系,检出限为1.7×10-11mol/L,D-甘露糖和ConA之间的结合常数为2.6×106L/mol。该方法简单,可适用于不同糖和蛋白质相互作用的研究,为构建高灵敏度的电化学阻抗传感器提供了新思路。
[Abstract]:The interaction between D-mannose and concanavalin (ConA) was studied. D-mannose covalent binding to the surface of loaded protein bovine serum protein (BSA) was carried out by Maillard reaction to form glycosylated protein. Then the glycosylated protein was immobilized on the surface of glassy carbon electrode, and the electrochemical impedance sensor for the detection of concanavalin (ConA) was constructed using D-mannose on the surface of glycosylated protein as molecular recognition material. The preparation process of glycosylated protein is simple, the loading of D-mannose is large, and it provides many binding sites in space, so it can form multivalent complex with ConA and improve the sensitivity of the sensor. The linear relationship between the response value of the sensor and the logarithm of ConA concentration was found in the range of 5.0 脳 10 ~ (-11) mol / L ~ 5.0 脳 10-9mol/L. The detection limit was 1.7 脳 10 ~ (-11) mol / L ~ (-1) -mannose and the binding constant between ConA and ConA was 2.6 脳 10 ~ (-6) L / mol. The method is simple and suitable for the study of the interaction between different carbohydrates and proteins, and provides a new idea for the construction of electrochemical impedance sensors with high sensitivity.
【作者单位】： 宁夏师范学院化学化工学院;固原市原州区黄铎堡学校;
【基金】：宁夏师范学院重点项目(NX SFZD1602) 六盘山工程中心项目(HG16-06)
【分类号】：O657.1;TP212.3
本文编号：2422512
[Abstract]:The interaction between D-mannose and concanavalin (ConA) was studied. D-mannose covalent binding to the surface of loaded protein bovine serum protein (BSA) was carried out by Maillard reaction to form glycosylated protein. Then the glycosylated protein was immobilized on the surface of glassy carbon electrode, and the electrochemical impedance sensor for the detection of concanavalin (ConA) was constructed using D-mannose on the surface of glycosylated protein as molecular recognition material. The preparation process of glycosylated protein is simple, the loading of D-mannose is large, and it provides many binding sites in space, so it can form multivalent complex with ConA and improve the sensitivity of the sensor. The linear relationship between the response value of the sensor and the logarithm of ConA concentration was found in the range of 5.0 脳 10 ~ (-11) mol / L ~ 5.0 脳 10-9mol/L. The detection limit was 1.7 脳 10 ~ (-11) mol / L ~ (-1) -mannose and the binding constant between ConA and ConA was 2.6 脳 10 ~ (-6) L / mol. The method is simple and suitable for the study of the interaction between different carbohydrates and proteins, and provides a new idea for the construction of electrochemical impedance sensors with high sensitivity.
【作者单位】： 宁夏师范学院化学化工学院;固原市原州区黄铎堡学校;
【基金】：宁夏师范学院重点项目(NX SFZD1602) 六盘山工程中心项目(HG16-06)
【分类号】：O657.1;TP212.3
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1 张健,张其胜,田庚元;拟糖蛋白合成研究进展[J];有机化学;2003年05期
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