2015年北京市昌平区副溶血性弧菌临床菌株分子特征及耐药性研究

发布时间：2018-01-06 17:17

本文关键词：2015年北京市昌平区副溶血性弧菌临床菌株分子特征及耐药性研究　出处：《现代预防医学》2017年04期 　论文类型：期刊论文

【摘要】：目的了解2015年昌平区副溶血性弧菌临床菌株耐药状况及分子特征。方法药敏实验按照美国临床实验室标准化协会(CLSI)推荐的K-B纸片法进行24种抗生素的敏感性分析;PFGE实验参照Pulse Net脉冲场凝胶电泳实验方法。结果药敏结果显示,副溶血性弧菌对阿米卡星和妥布霉素完全耐药,对萘替米星耐药率高达95.45%,对米诺环素和卡那霉素处于耐受和敏感之间,对氨苄西林和头孢呋新钠耐药或介于耐药和敏感之间。耐药谱分析显示,副溶血性弧菌同时耐受3~7种抗生素。PFGE分析发现,22株副溶血性弧菌有17种PFGE带型,可分为2个聚类群,血清型O3∶K6属于A群且大部分菌株对氨苄西林、头孢呋新钠耐药。结论昌平区引起腹泻的副溶血性弧菌耐受抗生素的种类多、多重耐药严重且PFGE带型呈高度多态性。
[Abstract]:Objective to investigate the drug resistance and molecular characteristics of Vibrio parahaemolyticus strains in Changping District in 2015. The recommended K-B disk method was used to analyze the sensitivity of 24 antibiotics. PFGE experiment was based on the method of Pulse Net pulsed field gel electrophoresis. The results showed that Vibrio parahaemolyticus was completely resistant to amikacin and tobramycin. The drug resistance rate to naftilmicin was 95.45%, the resistance to minocycline and kanamycin was between tolerance and sensitivity, and the resistance to ampicillin and cefuroxime sodium was between resistance and sensitivity. Vibrio parahaemolyticus was also tolerant to 37 antibiotics. PFGE analysis showed that there were 17 PFGE bands in 22 strains of Vibrio parahaemolyticus, which could be divided into 2 clusters. The serotype O3: K6 belongs to group A and most of the strains are resistant to ampicillin and cefuroxime sodium. Conclusion there are many kinds of antibiotics resistant to Vibrio parahaemolyticus caused by diarrhea in Changping District. Multidrug resistance is severe and PFGE band type is highly polymorphic.
【作者单位】：北京市昌平区疾病预防控制中心微生物检验科;
【分类号】：R446.5
【正文快照】： 副溶血性弧菌是一种革兰阴性嗜盐菌,广泛存在于鱼虾、贝类等海产品中,是我国沿海地区食物中毒和急性腹泻的主要病原菌[1-3]。随着海产品消费大众化,内地地区由副溶血性弧菌引起的食物中毒和急性腹泻病例增加,副溶血性弧菌已成为我国重要的食源性病原菌[4-7]。本研究对2015年从

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