磁共振DTI定量评价脉络宁注射液对非压迫性腰神经根炎疗效的实验研究

发布时间：2018-01-08 03:00

本文关键词：磁共振DTI定量评价脉络宁注射液对非压迫性腰神经根炎疗效的实验研究　出处：《重庆医学》2017年21期 　论文类型：期刊论文

【摘要】：目的探索运用磁共振扩散张量成像(MR-DTI)定量评价脉络宁注射液对非压迫性腰神经根炎的疗效。方法选取巴马小型猪9只,分入A组、B组、C组,各3只,在CT引导下建立非压迫性腰神经根炎模型,各组于造模后14d硬膜外分别注射脉络宁(10mL)、生理盐水(10mL)、消炎液(10mL),造模前、造模后14d、治疗前均进行MR-DTI扫描,治疗后3、7、14d分别从各组取出1只实验猪进行MR-DTI扫描后处死并取神经根标本进行免疫组织化学检测,在MR-DTI图像中测量神经根各向异性分数(FA)值,对FA值和免疫组织化学结果进行统计学分析。结果 MR-DTI:各组造模后FA值均降低(P0.05);A组治疗后14dFA值回升,与治疗前比较,差异有统计学意义(P0.05);A组治疗后3、7dFA值无明显上升,A、B两组间比较,差异无统计学意义(P0.05),但C组FA值回升较其他两组早且迅速(P0.05);治疗后14d,A组FA值回升,双侧神经根FA值分别与B组、C组比较,差异均无统计学意义(P0.05),而B组和C组间FA值比较差异有统计学意义(P0.05)。免疫组织化学:治疗后7d,A组神经组织肿瘤坏死因子α(TNF-α)的积分吸光度值(IA值)开始下降;治疗后14d,A组与C组TNF-αIA值较B组明显降低,差异有统计学意义(P0.05)。结论脉络宁注射液对非压迫性腰神经根炎具有一定疗效,并可用DTI对其进行评价。
[Abstract]:Objective to evaluate quantitatively the efficacy of Mailuoning injection in the treatment of non-compressive lumbar radiculitis by using Mr diffusive Zhang Liang MR-DTI. Methods Nine Bama miniature pigs were selected and divided into group A and group B, respectively. The model of non-compressive lumbar radiculitis was established in each group under the guidance of CT. The rats in each group were injected with Mailuoning 10mLL and normal saline 10mL respectively on the 14th day after the establishment of the model. The MR-DTI scan was performed before and 14 days after the treatment of Xiaoyan liquid (10 mL / L), and 3 days after treatment. On the 14th day, one pig was removed from each group for MR-DTI scanning, and then the nerve root samples were collected for immunohistochemical examination. Nerve root anisotropy fraction (FAA) was measured in MR-DTI images. Results the results of MR-DTI showed that the FA value of each group was lower than that of the control group (P 0.05). The FA value of group A increased at 14 days after treatment, and the difference was statistically significant compared with that before treatment (P 0.05). There was no significant increase in FA value in group A at 3 ~ 7 days after treatment. There was no significant difference between the two groups, but the FA value in group C increased earlier than that in the other two groups (P 0.05). After 14 days of treatment, the FA value of group A was increased, and the FA value of bilateral nerve roots was not significantly different from that of group B and group C (P 0.05). The difference of FA value between group B and group C was statistically significant (P 0.05). Immunohistochemistry: 7 days after treatment. In group A, the integral absorbance of TNF- 伪 in nerve tissue began to decrease. The value of TNF- 伪 IA in group A and group C was significantly lower than that in group B on the 14th day after treatment, and the difference was statistically significant (P 0.05). Conclusion Mailuoning injection has a certain curative effect on non-compressive lumbar radiculitis. It can be evaluated by DTI.
【作者单位】：成都大学附属医院放射科;
【基金】：四川省医学科研青年创新课题计划项目(Q14004)
【分类号】：R445.2;R681
【正文快照】： 腰椎间盘髓核突出引起的化学性炎性反应是引起腰神经根性疼痛的重要因素[1]。硬膜外类固醇药物注射已成为目前治疗腰神经根痛的重要方式,但其在临床适应证、有效性和风险方面仍存在较大的争议,因此其他药物的疗效受到越来越多的关注[2]。据文献报道,脉络宁注射液代替激素治疗

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