成都地区1040份痰液人鼻病毒的分离和分型

发布时间：2018-01-08 03:32

本文关键词：成都地区1040份痰液人鼻病毒的分离和分型　出处：《现代预防医学》2017年18期 　论文类型：期刊论文

【摘要】：目的探讨四川省成都地区发热呼吸道住院患者人鼻病毒(HRV)感染的分子流行病学特征,为本地人鼻病毒感染的防控积累资料。方法于2014-2016年采集成人发热呼吸道症候群住院患者痰液1 040份,提取标本核酸,利用逆转录聚合酶链式反应(RT-PCR)以及巢式PCR(Nested-PCR)扩增HRV-5'NCR基因,筛查HRV阳性标本,随机选取61份阳性标本扩增VP4/VP2基因,测定核酸序列并进行同源性比对和分型。结果 1 040例标本核酸检出率为10.9%;冬春季检出率高,随年龄升高检出率呈上升趋势;获得的61条VP4/VP2序列中,30条为HRV-A型,10条为HRV-B型,21条为HRV-C型;并发现HRV基因型内和型间核酸同源性差异均较大。结论成都地区成人发热呼吸道症候群住院患者HRV的感染率高,以A型与C型流行为主,病毒VP4/VP2核酸片段变异较大。
[Abstract]:Objective to investigate the molecular epidemiological characteristics of human rhinovirus (HRV) infection in hospitalized patients with fever and respiratory tract in Chengdu, Sichuan Province. Methods from 2014 to 2016, 1 040 samples of sputum from adult patients with febrile respiratory syndrome were collected and their nucleic acids were extracted. The HRV-5'NCR gene was amplified by reverse transcriptase polymerase chain reaction (RT-PCR) and nested PCR-Nested-PCR. HRV positive samples were screened. VP4/VP2 gene was amplified from 61 positive samples randomly, nucleic acid sequence was determined, homologous alignment and typing were carried out. Results the detection rate of nucleic acid was 10.9% in 1 040 samples. The detection rate was high in winter and spring and increased with age. Among the 61 VP4/VP2 sequences, 30 were HRV-A and 10 were HRV-B. 21 were HRV-C. It was also found that the homology of nucleic acid in and between HRV genotypes was significant. Conclusion the infection rate of HRV in adult patients with febrile respiratory syndrome in Chengdu area is high, mainly in type A and type C. VP4/VP2 nucleic acid fragments of the virus vary greatly.
【作者单位】：四川大学华西公共卫生学院(华西第四医院);
【基金】："十二五"国家科技重大专项云南及周边省市区传染病病源谱流行规律研究(2012ZX10004212-002)
【分类号】：R440;R511
【正文快照】： 人鼻病毒(Human Rhinovirus,HRV)属于小核糖核酸病毒科肠道病毒属单正链RNA病毒,是常见的呼吸道感染病原体。基因全长7 200 bp,编码4个蛋白和7个非结构蛋白,其中HRV-5‘NCR为人鼻病毒多聚蛋白基因,此片段具有高保守性,高特异度及检测灵敏度,因此,作为人鼻病毒感染初筛的目标基

【相似文献】