基于铜离子修饰金纳米簇“关-开”型荧光探针检测多巴胺

发布时间：2018-01-13 11:39

本文关键词：基于铜离子修饰金纳米簇“关-开”型荧光探针检测多巴胺　出处：《高等学校化学学报》2017年12期 　论文类型：期刊论文

【摘要】：以11-巯基十一烷酸(11-MUA)为还原剂和保护剂,通过一步水热法合成了具有强烈荧光的水溶性金纳米簇(AuNCs),基于Cu~(2+)修饰的AuNCs@11-MUA构建了"关-开"型荧光探针用于多巴胺(DA)的选择性、高灵敏检测.向AuNCs@11-MUA溶液中加入Cu~(2+)离子后,AuNCs@11-MUA的荧光发生猝灭,体系的荧光信号处于"关闭"状态.在DA存在下,由于DA与Cu~(2+)具有更强的结合力,形成比Cu~(2+)/AuNCs@11-MUA复合体更稳定的络合物,可将Cu~(2+)从AuNCs@11-MUA表面移除下来,从而使其荧光得以恢复,体系的荧光信号呈"打开"状态.AuNCs@11-MUA探针的荧光恢复程度与DA的浓度在2.0×10~(-7)~5.0×10~(-5)mol/L范围呈良好的线性关系,检出限为8.0×10~(-8)mol/L(S/N=3).将该探针应用于人血清和尿液中DA的检测,回收率为93.2%~97.3%,相对标准偏差RSD4.08%,表明该方法可应用于人体内多巴胺的检测.
[Abstract]:Using 11-mercaptodecanoic acid (11-MUA) as reductant and protectant, water-soluble gold nanoclusters (AuNCswith strong fluorescence) were synthesized by one-step hydrothermal method. The selectivity of "turn-open" fluorescent probe for dopamine was constructed based on AuNCs@11-MUA modified by Cu~(2. The fluorescence of AuNCs@ 11-MUA was quenched after the addition of Cu~(2 ion into AuNCs@11-MUA solution. The fluorescence signal of the system is in a "off" state. In the presence of DA, there is a stronger binding force between DA and Cu~(2. The formation of a more stable complex than the Cu~(2 / AuNCs@ 11-MUA complex can remove Cu~(2 from the AuNCs@11-MUA surface. So that its fluorescence can be restored. The fluorescence signal of the system was "open". The fluorescence recovery degree of the AuNCs@ 11-MUA probe and the concentration of DA were 2.0 脳 10 ~ (-7) and 5.0 脳 10 ~ (10) ~ (-5)). The range of mol/L is linear. The detection limit was 8.0 脳 10 ~ (-1) ~ (-8) mol / L ~ (-1) N ~ (-1). The probe was applied to the detection of DA in human serum and urine. The recovery rate was 93.22% ~ 97.3%. The relative standard deviation (RSD) of RSD 4.08 indicates that this method can be applied to the detection of dopamine in human body.
【作者单位】：青岛科技大学化学与分子工程学院生态化工教育部重点实验室;
【基金】：国家自然科学基金(批准号:21575073) 山东省自然科学基金(批准号:ZR2013BQ004) 山东省高等学校科技计划项目(批准号:J15LC14)资助~~
【分类号】：O657.3;R446.1
【正文快照】： 金纳米簇(Au NCs)由几十至几百个金原子构成,其粒径通常小于2 nm.由于粒径接近电子的费米波长,因此Au NCs具有与大粒径金纳米颗粒不同的光学、化学和电学性质.作为一种新型荧光纳米材料,金纳米簇具有合成简单、光稳定性强和生物兼容性好等优点,目前已经成为纳米领域中发展前景

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