原发性胆汁性肝硬化患者IgG型自身抗体检测的临床价值

发布时间：2018-01-13 13:32

本文关键词：原发性胆汁性肝硬化患者IgG型自身抗体检测的临床价值　出处：《检验医学与临床》2017年02期 　论文类型：期刊论文

【摘要】：目的分析原发性胆汁性肝硬化(PBC)患者的自身免疫性肝病抗体谱及抗核抗体荧光核型,探讨其在PBC诊断及临床应用中的意义。方法选择已确诊的PBC患者67例及病例对照组70例,检测自身免疫性肝病抗体谱,间接免疫荧光法检测抗核抗体,应用SPSS17.0统计软件进行分析处理。结果 PBC患者组阳性检出率以AMA-M2抗体、Sp100抗体及gp210抗体为主,抗核抗体荧光核型以核点型和胞浆颗粒型为主。AMA-M2抗体、Sp100抗体和gp210抗体在PBC患者的阳性率显著高于对照组(χ2=37.228,5.492和16.292,P0.01);而LKM1抗体、LC1抗体、SLA抗体阳性率与对照组比较,差异无统计学意义(P0.05)。结论自身免疫性肝病抗体谱,特别是血清AMA-M2阳性是诊断PBC的重要指标之一,另外Sp100抗体、gp210抗体也是PBC诊断的特征性抗体,其对AMA-M2阴性PBC的诊断具有重要作用。
[Abstract]:Objective to analyze the autoimmune liver disease antibody spectrum and the fluorescence karyotype of antinuclear antibody in patients with primary biliary cirrhosis (PBC). Methods 67 cases of PBC and 70 cases of control group were selected to detect the antibody spectrum of autoimmune liver disease. Indirect immunofluorescence assay was used to detect the antinuclear antibody and SPSS17.0 software was used to analyze it. Results the positive rate of PBC patients was AMA-M2 antibody. The Sp100 antibody and gp210 antibody were dominant, and the fluorescent karyotype of antinuclear antibody was nuclear point type and cytoplasmic granule type. AMA-M2 antibody was the main type. The positive rates of Sp100 antibody and gp210 antibody in PBC patients were significantly higher than those in the control group (蠂 ~ 2 + 37.22 28 ~ 5.492 and 16.292% P _ (0.01)). However, there was no significant difference in the positive rate of LKM1 antibody against LC1 antibody and SLA antibody between the control group and the control group. Conclusion the antibody spectrum of autoimmune liver disease is not significantly different from that of the control group (P < 0.05). In particular, the positive serum AMA-M2 is one of the important indexes in the diagnosis of PBC. In addition, the Sp100 antibody, gp210, is also the characteristic antibody in the diagnosis of PBC. It plays an important role in the diagnosis of AMA-M2 negative PBC.
【作者单位】：昆明医科大学附属延安医院医学检验科;昆明医科大学附属延安医院消化科;
【基金】：云南省科技厅-昆明医科大学联合专项项目(2015FB086) 昆明医科大学研究生创新基金资助项目(2016S43)
【分类号】：R575.22;R446.6
【正文快照】： 原发性胆汁性肝硬化(PBC)是一种病因和发病机制尚不完全清楚的慢性进行性胆汁淤积性肝脏病变,病理表现为肝内胆管非化脓性炎性伴有小叶间胆管破坏、门静脉周围炎和肝实质碎屑样坏死,最终进展为肝硬化[1]。该病多见于中年女性,患者血清中常常出现抗线粒体抗体,此自身抗体是PBC

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