醒脑静注射液对脓毒症大鼠心肌酶和线粒体呼吸链复合物的作用

发布时间：2018-02-14 20:31

  本文关键词： 醒脑静注射液 脓毒症 心肌酶 线粒体呼吸链复合物　出处：《中国临床药理学杂志》2017年07期 　论文类型：期刊论文

【摘要】：目的研究醒脑静注射液对脓毒症大鼠心肌酶和线粒体呼吸链复合物的影响。方法按照体重将SD大鼠随机分为3组:正常组10只,腹腔注射与脂多糖(LPS)等量的0.9%NaCl;其余SD大鼠腹腔注射10 mg·kg~(-1)脂多糖(LPS),建立脓毒症模型。模型组与实验组均30只,实验组经胃管注入醒脑静注射液6.4 mL·kg~(-1)。于给药后6,24,48 h,用试剂盒法检测不同时点各组的心肌酶和呼吸链复合物的活性,包括NADH-辅酶Q还原酶(complexⅠ)、琥珀酸-辅酶Q还原酶(complexⅡ)、辅酶Q-细胞色素C还原酶(complexⅢ)、细胞色素C-氧化还原酶(complexⅣ)。结果在24 h,实验组与模型组的血清肌酸激酶分别为(1333.20±140.87),(1813.50±243.72)U·L~(-1);这2组的肌酸激酶同工酶分别为(748.15±119.02),(1167.92±145.17)U·L~(-1)。实验组的肌酸激酶、肌酸激酶同工酶水平均显著低于模型组,差异均有统计学意义(P0.01)。在24 h,实验组和模型组的心肌线粒体complexⅠ活性分别为(2.83±0.15),(1.84±0.18)u·mg~(-1),这2组的complexⅢ活性分别为(0.91±0.13),(0.68±0.16)u·mg~(-1),这2组的complexⅣ分别为(19.43±1.37),(15.20±1.79)u·mg~(-1),实验组心肌线粒体complexⅠ、Ⅲ、Ⅳ活性均显著高于模型组差异均有统计学意义(P0.05)。结论醒脑静注射液可显著降低脓毒症大鼠心肌酶水平,提高脓毒症大鼠心肌线粒体complexⅠ、Ⅲ及Ⅳ活性。
[Abstract]:Objective to study the effect of Xingnaojing injection on myocardial enzyme and mitochondrial respiratory chain complex in septic rats. Methods SD rats were randomly divided into 3 groups according to their body weight: 10 normal rats. The sepsis model was established by intraperitoneal injection of 0.9% NaCl1 to lipopolysaccharide (LPS) and intraperitoneal injection of 10 mg 路kg ~ (-1) lipopolysaccharide (LPS-1) into the other SD rats. Xingnaojing injection (6.4 mL 路kg ~ (-1)) was injected into the stomach tube of the experimental group. The activity of myocardial enzyme and respiratory chain complex at different time points were detected by kit method at 6 ~ 24m ~ 48 h after administration. These include NADH- coenzyme Q reductase complex 鈪，

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