TRIzol法与磁珠法用于丙型肝炎病毒RNA定量检测的比较

发布时间：2018-03-04 22:34

本文选题：丙型肝炎病毒　切入点：TRIzol法　出处：《第二军医大学学报》2017年07期 　论文类型：期刊论文

【摘要】：目的比较TRIzol法与磁珠法对丙型肝炎病毒(HCV)RNA定量检测结果的影响。方法收集117例丙型肝炎病毒感染阳性患者的血清及其基因分型信息。分别采用TRIzol法与磁珠法提取患者血清样本的HCV RNA,qPCR检测提取HCV RNA的病毒载量,分析两种提取方法所得HCV RNA检测结果的差异。结果 qPCR结果显示TRIzol法和磁珠法具有良好的线性相关性:y=0.978x+0.063(R2=0.973)。Bland-Altman统计分析显示TRIzol法提取的HCV RNA病毒载量对数值的平均值略低于磁珠法,但差异无统计学意义(P0.05)。基因型分析结果显示1a、1b、2a、3a和6a基因型中两种提取方法差异均无统计学意义(P0.05)。结论 TRIzol法提取HCV RNA的定量检测结果和磁珠法相当,且成本低廉,可在国内广泛用于试剂盒的开发和HCV RNA临床检测。
[Abstract]:Objective to compare the effect of TRIzol method and magnetic beads method on the quantitative detection of hepatitis C virus (HCV) RNA. Methods the serum and genotyping information of 117 patients with hepatitis C virus infection were collected and extracted by TRIzol method and magnetic beads method respectively. The viral load of HCV RNA extracted from patients' serum samples was detected by HCV RNA-qPCR. Results the qPCR results showed that there was a good linear correlation between the TRIzol method and the magnetic bead method. The statistical analysis showed that the average value of the HCV RNA virus load extracted by TRIzol method was slightly lower than that by the magnetic beads method, and the statistical analysis showed that there was a good linear correlation between the TRIzol method and the magnetic bead method, and the statistical analysis showed that the HCV RNA virus load extracted by TRIzol method was slightly lower than that by the magnetic bead method. The results of genotype analysis showed that there was no significant difference between the two methods for extracting HCV RNA from 1a and 6a genotypes. Conclusion the quantitative detection results of HCV RNA by TRIzol method are comparable to those by magnetic beads method and the cost is low. It can be widely used in the development of kit and clinical detection of HCV RNA in China.
【作者单位】：福建医科大学附属第二医院检验科;
【分类号】：R440;R512.63

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