降钙素原、人中性粒细胞载脂蛋白和中性粒细胞CD64检测在细菌性感染早期诊断中的应用

发布时间：2018-03-07 18:40

本文选题：感染　切入点：降钙素原　出处：《中国感染与化疗杂志》2017年06期 　论文类型：期刊论文

【摘要】：目的研究细菌性感染患者血液中的降钙素原(PCT)、人中性粒细胞载脂蛋白(HNL)和中性粒细胞CD64(CD64)的表达水平,为临床细菌性感染早期诊疗提供科学依据。方法选取2013年2月—2017年5月就诊治疗确诊的210例感染患者,另选取80例同期健康体检人员为对照组,感染患者分为细菌组105例、病毒组105例,采用热景公司上转发光免疫分析仪检测PCT和HNL、BD FACS calibur流式细胞仪测定并计算CD64百分比、制定工作曲线选取PCT、HNL及CD64截断值,从而分析比较细菌组、病毒组和对照组的PCT、HNL及CD64表达水平和诊断细菌性感染疾病的特异度和灵敏度。结果细菌组与病毒组、对照组的PCT、HNL、CD64和WBC水平分别比较,差异均具有统计学意义(P0.05)。病毒组与对照组的CD64、WBC水平比较,差异均具有统计学意义(P0.05)。病毒组与对照组的PCT和HNL水平差异无统计学意义(P0.05)。PCT的受试者操作特征(ROC)曲线下的面积(AUC)为0.855,AUC(HNL)为0.930,AUC(CD64)为0.928,AUC(WBC)为0.729。PCT、HNL和CD64诊断细菌感染性疾病的截断值分别为:?0.79 ng/m L、87.43 ng/m L、9.01%。结论细菌感染性疾病导致PCT、HNL和CD64表达水平升高,PCT、HNL和CD64可用于细菌感染性疾病的早期诊断,其中HNL的诊断价值最高。
[Abstract]:Objective to study the expression of procalcitonin (PCT), human neutrophil apolipoprotein (apolipoprotein) (HNL) and neutrophil CD64 (CD64) in the blood of patients with bacterial infection. Methods from February 2013 to May 2017, 210 patients with bacterial infection were selected and 80 healthy persons were selected as control group. The infected patients were divided into bacterial group (105 cases), bacterial group (105 cases). In 105 cases of virus group, PCT and HNLBD FACS calibur were detected and the percentage of CD64 was calculated by flow cytometry, and the working curve was selected to select the truncation values of HNL and CD64 to analyze and compare the bacterial group. Results the levels of PCTNLLCD64 and WBC were compared between the viral group and the control group, and the specificity and sensitivity in the diagnosis of bacterial infection. The difference was statistically significant (P 0.05). The level of CD64 WBC in the virus group was compared with that in the control group. There was no significant difference in the levels of PCT and HNL between the virus group and the control group. There was no significant difference in the PCT and HNL levels between the virus group and the control group. The area of AUC under the operating characteristics curve was 0. 855AUC HNL (0. 930 AUC AUC WBC64) was 0. 928 AUCWBCC) and the truncation value of CD64 was 0. 729. PCT HNL and CD64 for the diagnosis of bacterial infectious diseases, respectively. Conclusion the expression level of CD64 and HNL of PCT was increased due to bacterial infectious diseases. HNL and CD64 can be used in the early diagnosis of bacterial infectious diseases, and HNL is the most valuable in the diagnosis of bacterial infectious diseases.
【作者单位】：衡水市哈励逊国际和平医院检验科;河北省衡水市妇幼保健院检验科;
【基金】：河北省衡水市科学技术研究与发展计划(2017014014Z)
【分类号】：R446.1;R515

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