耐万古霉素肠球菌临床菌株毒力基因携带情况及差异

发布时间：2018-03-07 18:42

本文选题：耐万古霉素肠球菌　切入点：屎肠球菌　出处：《中国人兽共患病学报》2017年05期 　论文类型：期刊论文

【摘要】：目的检测不同种类耐万古霉素肠球菌(VRE)毒力基因携带情况及其差异,为研究VRE致病机制提供基础。方法采用微量稀释法检测浙江地区490株屎肠球菌和862株粪肠球菌临床分离菌株对万古霉素的耐药性。采用PCR检测VRE菌株ace、asa1、cylA、efaA、esp、gelE、hyl七种毒力基因。结果 10%(49/490)屎肠球菌株和0.8%(7/862)粪肠球菌株为VRE。万古霉素耐药屎肠球菌株中,5株未检出任何毒力基因,其余44株仅可检出asa1、esp、gelE和hyl基因,其中esp基因(73.5%,36/49)和hyl基因(53.1%,26/49)为优势基因,以单或双基因为主要携带模式。万古霉素耐药粪肠球菌株中可检出除hyl基因以外其他6种毒力基因,且每株菌均同时携带3~6种致病基因。结论本地区VRE以屎肠球菌为主,耐万古霉素屎肠球菌和粪肠球菌毒力基因携带率、携带种类和模式均有明显差异。
[Abstract]:Objective to detect the virulence gene carriers of different vancomycin resistant enterococci and their differences. Methods the resistance of 490 strains of Enterococcus faecium and 862 strains of Enterococcus faecalis to vancomycin was detected by microdilution method. PCR was used to detect the seven virulence of VRE strain. Results Enterococcus faecium strain and Enterococcus faecalis strain were VRE.Virulence genes were not detected in 5 strains of vancomycin resistant Enterococcus faecium strain. In the remaining 44 strains, only ASA 1 EPG E and hyl genes were detected, of which the esp gene was 73.536 / 49) and the hyl gene was 53.1c26 / 49) as the dominant gene, with a single or double gene as the main carrier. Six virulence genes other than the hyl gene could be detected in the vancomycin resistant Enterococcus faecalis strain. Conclusion Enterococcus faecium is the main virulence gene of VRE in this region, and the virulence gene carrying rate of vancomycin resistant Enterococcus faecalis and Enterococcus faecalis are significantly different.
【作者单位】：浙江省人民医院检验中心;浙江省新华医院;
【基金】：浙江省医药卫生科技计划项目(2017KY004)资助~~
【分类号】：R446.5

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