血液分析仪免疫比浊法检测C反应蛋白临床应用

发布时间：2018-03-18 14:43

本文选题：全自动血液分析仪　切入点：C-反应蛋白　出处：《临床军医杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的探讨BC-5390血液分析仪免疫比浊法检测全血C反应蛋白(CRP)的临床应用。方法收集2015年3—6月德阳市人民医院就诊的患者和健康体检人员各20例EDTA抗凝全血标本,根据美国临床和实验室标准化协会(CLSI)的要求,结合实际工作对仪器的分析性能进行评价。结果 BC-5390全自动血细胞分析仪(简称BC-5390血液分析仪)检测CRP批内精密度变异系数(CV)值为2.01%,批间精密度两个浓度CV值分别为2.76%和2.70%。仪器检测线性验证回归方程:Y=0.983X+4.814,r~2=0.995。20例健康体检人员的CRP检测结果均在参考范围0~4 mg/L。BC-5390血液分析仪和QuikRead go分析仪检测全血CRP结果进行对比分析,回归方程:Y=0.833X-3.271,r~2=0.991。室温或冷藏条件保存的抗凝血标本24 h内,结果稳定性良好,相对偏差3%。BC-5390血液分析仪检测CRP携带污染符合相应要求。结论 BC-5390血液分析仪测定全血标本的CRP性能验证指标合格,能够满足临床需求。
[Abstract]:Objective to investigate the clinical application of BC-5390 hematology analyzer in the detection of whole blood C-reactive protein (C-reactive protein). Methods 20 cases of EDTA anticoagulant whole blood samples were collected from the people's Hospital of Deyang City from 2015 to June. In accordance with the requirements of the American Association for Clinical and Laboratory Standardization, Results BC-5390 automatic blood cell analyzer (BC-5390 blood analyzer) was used to detect the intra-batch precision variation coefficient (CV) of CRP. The CV value of two concentrations of precision between batches was 2. 01%. The results of CRP detection in 0 ~ 4 mg/L.BC-5390 blood analyzer and QuikRead go analyzer were compared and analyzed in the reference range of 0 ~ 4 mg/L.BC-5390 blood analyzer and QuikRead go analyzer. The regression equation was 0.991.The stability of anticoagulant samples preserved at room temperature or in cold storage condition was good within 24 hours. The relative deviation of BC-5390 blood analyzer for the detection of CRP contamination accords with the corresponding requirements. Conclusion the CRP performance of whole blood samples measured by BC-5390 hematology analyzer is up to standard and can meet the clinical requirements.
【作者单位】：德阳市人民医院检验科;
【分类号】：R446.1

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