耐碳青霉烯类肠杆菌属细菌的耐药基因分型及同源性分析

发布时间：2018-04-23 02:40

本文选题：碳青霉烯类耐药 + 肠杆菌属　；参考：《实用医学杂志》2017年19期

【摘要】：目的了解分离自我院住院患者的耐碳青霉烯类肠杆菌属细菌的耐药基因型及其同源性,为临床治疗提供理论依据。方法用Vitek-2 compact检测细菌对临床常用抗菌药物的药物敏感性;用PCR方法检测30株耐碳青霉烯类肠杆菌的6种耐药基因bla KPC、bla NDM-1、bla IMI-1、bla GES、bla SME和bla SHV。以Eric-PCR对5株bla NDM-1阳性者进行同源性检测。结果碳青霉烯类耐药肠杆菌属细菌30株,其中阴沟肠杆菌17株,产气肠杆菌10株,坂崎肠杆菌2株,生癌肠杆菌1株。检出的阳性基因分别为bla SHV 6株(20.0%)、bla NDM-1 5株(16.7%)、bla KPC 2株(6.7%)和bla GES 1株(3.3%)。5株bla NDM-1阳性菌株可以分为3个型。结论本次实验检测出的耐碳青霉烯类肠杆菌属,其耐药基因主要为bla SHV,其次为bla NDM-1,另有少部分bla KPC和bla GES。检测基因可能存在病房和医院内的克隆传播。
[Abstract]:Objective to investigate the genotypes and homology of Enterobacterium carbapenems isolated from inpatients in our hospital, and to provide a theoretical basis for clinical treatment. Methods Vitek-2 compact was used to detect the drug sensitivity of bacteria to commonly used antibiotics, and 30 strains of bla KPC-bla NDM-1nbla IMI-1BLA Gesibla SME and bla SHV were detected by PCR method. The homology of 5 bla NDM-1 positive strains was detected by Eric-PCR. Results there were 30 strains of carbapenem resistant Enterobacter, of which 17 were Enterobacter cloacae, 10 were Enterobacter aerogenes, 2 were Enterobacter sakazakii and 1 was Enterobacter carcinogenes. The positive genes were identified as bla SHV (6 strains) and bla GES (6 strains) and bla GES (5 strains of bla NDM-1) and bla GES (3. 3%), respectively. The positive genes were 16. 7%, 16. 7% and 6. 7%, respectively, and bla GES 1 strain was 3. 3% and 3. 5% of bla NDM-1 positive strains could be divided into three types. Conclusion bla SHV, bla NDM-1, and a small number of bla KPC and bla GESs are the main resistant genes of Enterobacteriaceae to carbapenem. Detection genes may exist in wards and hospitals for cloning and transmission.
【作者单位】：潍坊市人民医院;
【基金】：潍坊市科学技术发展计划项目(编号:2014WS019)
【分类号】：R446.5

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