RAGE基因多态性与脓毒症易感性研究

发布时间：2018-05-03 12:09

本文选题：脓毒症 + RAGE　；参考：《右江民族医学院学报》2017年01期

【摘要】：目的研究脓毒症患者与健康人晚期糖基化终末产物受体(RAGE)多态性分布规律,并探讨与脓毒症的关系。方法选择152例脓毒症患者(脓毒症组)及189例健康体检者(对照组)作为研究对象。应用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)技术对RAGE基因rs9469089、rs2070600、rs184003、rs2071288多态位点进行检测,并应用酶联免疫吸附法(ELISA)检测两组受试者血浆内皮素1(ET-1)水平,比较两组RAGE基因位点多态性分布规律,探讨其与脓毒症之间的关系。结果脓毒症组患者血浆ET-1水平显著高于对照组,差异有统计学意义(P0.05)。脓毒症组与对照组RAGE rs9469089、rs2070600、rs184003、rs2071288多态性位点的等位基因及基因型分布频率差异均无统计学意义(P0.05)。结论脓毒症患者血浆ET-1表达水平显著升高,所检测的RAGE基因多态性与脓毒症的易感性无相关。
[Abstract]:Objective to study the distribution of advanced glycosylation end product receptor (rag) polymorphism in patients with sepsis and healthy subjects, and to explore the relationship between the polymorphism and sepsis. Methods 152 patients with sepsis (sepsis group) and 189 healthy people (control group) were selected as study objects. Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) was used to detect the polymorphic loci of RAGE gene rs9469089, rs2070600, rs184003rs2071288, and the plasma endothelin 1ET-1 level was detected by Elisa. To investigate the relationship between RAGE locus polymorphism and sepsis. Results the plasma ET-1 level in sepsis group was significantly higher than that in control group (P 0.05). There was no significant difference in allele and genotype distribution of RAGE rs9469089, rs2070600, rs184003rs2071288 between sepsis group and control group (P 0.05). Conclusion the plasma ET-1 expression level was significantly increased in sepsis patients. The detected RAGE gene polymorphism was not associated with the susceptibility to sepsis.
【作者单位】：广西医科大学附属肿瘤医院麻醉科;右江民族医学院附属医院检验科;右江民族医学院附属医院消化科;右江民族医学院附属医院呼吸内科;右江民族医学院附属医院重症医学科;
【基金】：国家自然科学基金资助项目(81560321) 2014年广西医学高层次骨干人才培养“139”计划培养人选项目广西高校急重症分子免疫研究重点实验室项目(kfkt20160061)
【分类号】：R459.7

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