HNA-3在中国3个民族中的分布

发布时间：2018-05-20 00:43

本文选题：人类粒细胞抗原 + 测序　；参考：《中国输血杂志》2017年09期

【摘要】：目的研究四川汉族,西藏藏族和凉山彝族中HNA-3的分布频率,并比较产生抗-HNA-3a的理论风险。方法收集600例无偿献血员外周血标本,包括四川汉族献血者232例,西藏藏族献血者224例,凉山彝族献血者144例,采用PCR-SBT测序法对HNA-3进行分型。直接计数法计算基因型和等位基因频率。结果 HNA-3a和HNA-3b等位基因的频率分别在四川汉族人群为0.65和0.35,西藏藏族人群为0.66和0.34,凉山州彝族人群为0.60/0.40,3组之间没有统计学差异。基因型HNA-3a携带者(HNA-3a/a及HNA-3a/b)和HNA-3b/b分别在四川汉族人群为0.89和0.11,西藏藏族人群为0.88和0.12,凉山州彝族人群为0.81和0.19,汉族HNA-3b/b携带者显著低于彝族(P0.05)。结论彝族产生抗-HNA-3a的可能性比汉族和藏族大,汉族和藏族产生抗-HNA-3a的可能性相当,该数据为不同地区产生抗-HNA-3a的可能性提供一定数据支持。
[Abstract]:Objective to study the distribution frequency of HNA-3 in the Han, Tibetan and Liangshan Yi ethnic groups in Sichuan, and to compare the theoretical risk of anti-HNA-3a. Methods Peripheral blood samples were collected from 600 volunteer blood donors, including 232 blood donors from Sichuan Han nationality, 224 Tibetan blood donors from Tibet and 144 blood donors from Yi nationality in Liangshan. HNA-3 typing was performed by PCR-SBT sequencing. Genotypes and alleles were calculated by direct counting method. Results the frequencies of HNA-3a and HNA-3b alleles were 0.65 and 0.35 in Sichuan Han population, 0.66 and 0.34 in Tibetan nationality, and 0.60 / 0.40 in Yi nationality in Liangshan Prefecture, respectively. The genotypes of HNA-3a / a / a and HNA-3a / (b) and HNA-3b/b were 0.89 and 0.11 in Sichuan Han population, 0.88 and 0.12 in Tibetan population and 0.81 and 0.19 in Yi nationality in Liangshan Prefecture, respectively. The HNA-3b/b carriers in Han nationality were significantly lower than that in Yi nationality (P 0.05). Conclusion the probability of producing anti-HNA-3a in Yi nationality is greater than that in Han nationality and Tibetan nationality, and the probability of anti-HNA-3a in Han nationality and Tibetan nationality is similar. This data provides some data support for the possibility of anti--HNA-3a in different areas.
【作者单位】：中国医学科学院输血研究所临床输血研究中心;西藏自治区血液中心;凉山州中心血站;
【基金】：四川省科技厅项目资助(2017RZ0047) 中国医学科学院医学与健康科技创新工程资助(2016-I2M-3-024)
【分类号】：R440

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