基于便携拉曼光谱仪和双层纸芯片的头发皮质醇超高灵敏检测

发布时间：2018-05-28 06:59

本文选题：头发皮质醇 + 表面增强拉曼光谱　；参考：《化学学报》2017年04期

【摘要】：人头发中的皮质醇是反映现代人长期精神压力累积的主要临床标记物.建立了一种人发皮质醇的超高灵敏检测方法.方法基于表面增强拉曼光谱、免疫分析和双层纸芯片.纸芯片的第一层用于滤除人发提取液中的头发残渣(样品前处理),第二层用于实施竞争性免疫反应和拉曼检测.固定在纸表面的皮质醇抗原和游离的头发皮质醇竞争结合能产生拉曼信号的皮质醇单克隆抗体,通过检测结合在纸表面的皮质醇抗原-抗体复合物的拉曼信号进行游离头发皮质醇的定量.在便携式拉曼光谱仪上,皮质醇抗体的拉曼信号经过优化,方法的检测限可以达到1 pg/mL,平行测定相对标准偏差为8.38%(n=6).进行了两例实际样品检测,液质检测结果分别为0.771和0.153 ng/mL,本方法检测结果分别为0.63和0.247 ng/mL,两种方案的结果在一个数量级,证明了方法的实用性.利用此方法,一块芯片可以同时测定48个样品,专属性和准确性很好,特别适合于人群精神压力的普查.
[Abstract]:Cortisol in human hair is the main clinical marker reflecting long-term stress accumulation in modern people. A highly sensitive method for the detection of cortisol in human hair was established. Methods based on surface enhanced Raman spectroscopy, immunoassay and double-layer paper chip. The first layer of the paper chip is used to filter hair residue from the human hair extract (sample pretreatment) and the second layer is used to perform competitive immune reaction and Raman detection. The cortisol antigen fixed on the surface of the paper competes with the free hair cortisol to produce the cortisol monoclonal antibody, which produces the Raman signal. The free hair cortisol was measured by detecting the Raman signal of cortisol antigen-antibody complex on the paper surface. In a portable Raman spectrometer, the Raman signal of cortisol antibody is optimized, the detection limit of the method can reach 1 PG / mL, and the relative standard deviation of parallel determination is 8.38%. Two real samples were tested, the results of liquid quality detection were 0.771 and 0.153 ng / mL, respectively, and the results of this method were 0.63 and 0.247 ng / mL, respectively. The results of the two methods were of one order of magnitude, which proved the practicability of the two methods. By using this method, 48 samples can be determined simultaneously by a single chip. The specificity and accuracy of the method are very good, which is especially suitable for the general survey of mental stress in the population.
【作者单位】：大连理工大学制药科学与技术学院;大连化学物理研究所生物技术部;大连医科大学口腔医学院;
【基金】：国家自然科学基金(No.21675017)资助~~
【分类号】：R446.6;O657.37

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