胰腺癌LyP-1靶向性磁共振—荧光双模态分子探针实验

发布时间：2018-06-12 02:25

本文选题：胰腺肿瘤 + 分子探针　；参考：《中国医学影像技术》2017年10期

【摘要】：目的构建胰腺癌LyP-1靶向性磁共振—荧光双模态分子探针,观察其表征并进行MR显像。方法构建50nm核壳结构的磁共振—荧光双模态介孔探针,表面经环形多肽LyP-1(Cys-Gly-Asn-Lys-Arg-Thr-Arg-Gly-Cys)修饰,通过荧光及MR T2WI联合成像,验证该分子探针能否特异性识别并结合胰腺癌细胞。结果新型LyP-1靶向性磁共振—荧光双模态分子探针可用于小鼠原位胰腺癌的荧光显像和磁共振成像,可有效结合胰腺癌细胞并在体外使T2WI信号明显降低。体内实验表明,该分子探针可与荧光标记的胰腺癌组织靶向结合,并作为MRI对比剂显示C57BL/6小鼠原位胰腺癌。结论LyP-1靶向性磁共振—荧光双模态分子探针可靶向性地高效结合原位移植的小鼠胰腺癌细胞,用于早期胰腺癌诊断的转化研究。
[Abstract]:Objective to construct a LyP-1 targeted magnetic resonance fluorescence dual mode molecular probe for pancreatic cancer, and to observe its characterization and MR imaging. Methods the magnetic resonance fluorescence dual mode mesoporous probe of 50nm nuclear shell structure was constructed, the surface was trimed by circular polypeptide LyP-1 (Cys-Gly-Asn-Lys-Arg-Thr-Arg-Gly-Cys), and the fluorescence and MR T2WI were combined to verify the molecule. Can the probe specifically identify and combine pancreatic cancer cells. Results the novel LyP-1 targeted magnetic resonance fluorescence dual mode molecular probe can be used for fluorescence imaging and magnetic resonance imaging in mice in situ pancreatic cancer. It can effectively combine pancreatic cancer cells and reduce the T2WI signal in vitro. In vivo experiments show that the molecular probe can be used as a fluorescent marker. The targeted combination of pancreatic cancer tissue, and MRI contrast agent in C57BL/6 mice in situ pancreatic cancer. Conclusion LyP-1 targeted magnetic resonance fluorescence dual mode molecular probe can be targeted and efficiently combined with in situ transplanted pancreatic cancer cells for the early diagnosis of pancreatic cancer.
【作者单位】：第二军医大学附属长海医院放射科;复旦大学化学系;
【基金】：国家自然科学基金(81402680、81371551) 长海医院1255科学创新基金(CH125541000)
【分类号】：R445.2;R735.9

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